α – AMYLASE INHIBITORY ACTIVITY OF PARKIA BIGLOBOSA (MIMOSOIDEAE) SEED

Authors

  • Adediwura Fred-Jaiyesimi
  • Kio Abo
  • Richard Wilkins

Abstract

Parkia biglobosa (Jacq) Benth is a perennial legume tree grown in the Sudan and Guinea ecological Zones of West Africa. The fermented seed is used in West Africa to spice, flavour and thicken soups. Ethnomedically, it is reported as treatment for diabetes mellitus, snakebites, diarrhoeal infections, leprosy, hypertension and dental problems. Previous studies have reported the antidiarrhoeal, antihypertensive and antisnake venom activities. This bioactivity- guided study of Parkia biglobosa fermented seed using the alpha amylase inhibitory assay method adopted from Bernfeld (Bernfeld 1951; 1955) reports its activity. The methanol extract, its chloroform, chloroform/methanol and diethyl ether fractions were assayed for alpha amylase inhibitory potentials. The chloroform/methanol fraction exhibited a pronounced α – amylase inhibitory activity of 62% (p<0.05) comparable to that of Acarbose. The active fraction was subjected to column chromatography using solvents of varying polarities, an active sub-fraction obtained significantly inhibited the alpha amylase enzyme activity by 64% (P<0.05) compared to that of Acarbose (a known? – glucosidase inhibitor) which exhibited 59% inhibition. This study provides evidence that P. biglobosa and its fractions have ability to lower blood glucose by inhibiting the activity of the digestive enzyme, alpha amylase. Furthermore, it shows the nutraceutical potentials of Parkia biglobosa fermented seed (a West African spice). References: 1. Bernfeld, P (1951). Enzymes of starch degradation and synthesis in, Advances in Enzymology XII, F. Noed, Interscience publ. NY 379. 2. Bernfeld, P (1955). Methods in Enzymology 1, 149 – 158

Author Biography

Adediwura Fred-Jaiyesimi

Faculty of pharmacy, Oabisi Onabanjo University, Nigeria,

Published

2009-05-02

How to Cite

Fred-Jaiyesimi, A., Abo, K., & Wilkins, R. (2009). α – AMYLASE INHIBITORY ACTIVITY OF PARKIA BIGLOBOSA (MIMOSOIDEAE) SEED. African Journal of Traditional, Complementary and Alternative Medicines, 6, 390. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/769

Issue

ABSTRACTS OF THE WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS, CAPE TOWN NOVEMBER 2008

Section

Conference Abstracts

License

Copyright: Creative Commons Attribution CC.

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