TY - JOUR AU - Sari, Dwi Cahyani Ratna AU - Setyaningsih, Wiwit Ananda Wahyu AU - Mainichi, Rizky Nur AU - Dara, Gita Mumtarin AU - Kemalasari, Ratih AU - Romi, Muhammad Mansyur AU - Ar Rochmah, Mawaddah AU - Arfian, Nur PY - 2023/02/17 Y2 - 2024/03/29 TI - Centella Asiatica (GOTU KOLA) TREATMENT ATTENUATES PRO-INFLAMMATORY MEDIATORS IN LIVER OF RATS WITH ELECTRICAL FOOT SHOCK STRESS MODEL JF - African Journal of Traditional, Complementary and Alternative Medicines JA - Afr J Tradit Complement Altern Med VL - 17 IS - 2 SE - Research Papers DO - 10.21010/Ajtcamv17i2.1 UR - https://journals.athmsi.org/index.php/ajtcam/article/view/5321 SP - 1-7 AB - <p><strong>Background</strong>: Stress induces secretion of cathecolamines and glucocorticoids, which may produce liver injury. Followed by the production of inflammatory mediators, it causes apoptosis. <em>Centella asiatica</em> (CeA) has anti-inflammatory and hepatoprotective effects. The present study aims to determine the role of CeA in the attenuation of liver pro-inflammatory mediator expression in rats with electrical foot shock stress model.</p><p><strong>Materials and Methods</strong>: Twenty-four Sprague-Dawley rats were randomized into four groups consisted of six rats each: (1) Control group, (2) CeA-treated group, (3) Stress group, and (4) CeA + stress group. Reverse transcriptase PCR of inflammatory and apoptosis markers as well as Real-Time PCR of β2-adrenergic receptor were performed from liver tissues.</p><p><strong>Results</strong>: Electrical foot shock stress induced up-regulation of <em>NFκB</em> and <em>TNF-α</em> mRNA expressions as pro-inflammatory mediators, compared to control group. This alteration was followed by up-regulation of <em>BAX </em>and <em>β2-adrenergic receptor</em>, as well as the down-regulation of<em> BCl2</em> compared to control. CeA treatment prevented enhancement of <em>NFκB, TNF-α, TLR-4 </em>and<em> β-adrenergic receptor</em> mRNA expressions, which was followed by down-regulation of <em>BAX</em> and up-regulation of <em>BCl-2,</em> compared to stress group.</p><p><strong>Conclusion</strong>: CeA prevents secretion of pro-inflammatory chemokines and cytokines as well as apoptotic markers in liver cells through the activation of β2-adrenergic receptor.</p> ER -