BIOMARKERS AMONG TERPENOIDS AND QUANTITY OF EPIPHYTIC MICROFLORA OF ARTEMISIA L. SPECIES NATIVE FOR LITHUANIA
Abstract
Species belonging to Artemisia L. genus are rich in secondary metabolites (flavonoids, essential oils) and have been widely used in pharmacology and food industry. The concentration of the secondary metabolites depends on nutrition and developmental stage of the plant. Little is known about biology of Artemisia species (A. campestris L., A. vulgaris L. and A. absinthium L.) naturally growing in Lithuania. The aim of the work included evaluation of biomarkers among terpenoids in the plants, growing under different climatic and environmental conditions. Morphological properties of the plants were evaluated. For the quantity and chemical composition of the essential oils the following methods and techniques were used: gas chromatograph (HP 5890 II) with mass spectrometric detector (HP 5971), HPLC (chromatograph Waters 2690) equipped with VIS-UV detector (Waters 2487) and VIS-UV spectrophotometer (Genesys 5). The main biomarkers among terpenoids were determined (major specific compounds, with amount ³ 10 %): a- ir b -pinenes, sabinene, thujones, 1,8-cineole (for A. vulgaris), epoxi-ocimene (for A. absinthium), chrysanthenyl acetate (for A. vulgaris), trans-caryophyllene, germacrene D, trans-sabinyl acetate (for A. absinthium ), g-curcumene+acoradiene (for A. campestris). Synthesis of the secondary metabolites is influenced by nutrition and related to the quantity of the epiphytic microorganisms. According to the quantity of epiphytic microflora developmental differences were observed, also aboveground organ specificity was documented. No expressed differences were found between investigated species according to the amount of surface microorganisms. The differences in nitrogen nutrition got the best reflection in the ratio of the amounts of bacteria and fungi.Published
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