WIL-2 NS LYMPHOMA CELL LINE SHOW APOPTOTIC FEATURES WHEN TREATED WITH TRADITIONAL MEDICINE COMMELINA BENGHALESIS

Authors

  • M. P. Mokgotho
  • P. Masoko
  • V. G. Mbazima
  • K. W. Lebogo
  • L. J. Mampuru

Abstract

Many natural occurring agents have shown chemo preventive and chemotherapy potentials, yet there are no effective drugs to treat most cancers. Commelina benghalensis has been traditionally used as a herbal plant whose leaves are believed to have anti-tumour properties. The aim of this study was to investigate the effect of semi-purified extracts from C. benghalensis on the proliferation of Wil-2 NS cells. An acetone extract was prepared from the leaves and defatted with n-hexane and fractionated with dichloromethane (F2). F2 was tested for anti-proliferative activities on Wil-2 NS cells. Results from the trypan blue exclusion and sulphorhodamine B assays showed F2 inhibited the proliferation of the Wil-2 NS cells in a dose and time dependent manner. The acridine orange/ethidium bromide dual stain showed that the treated cells die through a process of apoptosis. These results show that C. benghalesis has anti-proliferation properties against Wil-2NS lymphoma cells. Further studies are required to determine the components responsible from the extracts and elucidate the mechanism of action on growth inhibition. Acknowledgements: NRF and University of Limpopo Research office.

Author Biography

M. P. Mokgotho

Biochemistry, Microbiology and Biotechnology Department, University of Limpopo, Sovenga, South Africa, Limpopo Province, 0727

Published

2009-05-03

How to Cite

Mokgotho, M. P., Masoko, P., Mbazima, V. G., Lebogo, K. W., & Mampuru, L. J. (2009). WIL-2 NS LYMPHOMA CELL LINE SHOW APOPTOTIC FEATURES WHEN TREATED WITH TRADITIONAL MEDICINE COMMELINA BENGHALESIS. African Journal of Traditional, Complementary and Alternative Medicines, 6, 394–395. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/778

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