BONE MINERALIZATION ENHANCING ACTIVITY OF A METHOXYELLAGIC ACID GLUCOSIDE FROM A FEIJOA SELLOWIANA LEAF EXTRACT

Abstract

As part of an ongoing study to discover potential bioactive phenolics from terrestrial plant sources [1,2], the capability of an aqueous methanol extract obtained from the leaves of Feijoa sellowiana Berg. on possible prevention and treatment of osteoporosis has been examined by evaluating its stimulant effect on the two human osteoblastic cell lines HOS58 and SaOS-2. The extract was found to increase significantly the mineralization of cultivated human bone cell, whereby a clear increment (15.3 ± 2.7%) in VON KOSSA positive area was determined when administering 25 ug/ml leaf extract. On the other hand a phytochemical investigation of the extract has demonstrated the high phenolic content and led to the isolation and identification of twenty three of them, among which the new 3-methoxyellagic acid 4-O-glucopyranoside was fully identified. All structures were elucidated on the basis of conventional analytical methods and confirmed by FTMS, 1D- and 2D-NMR data. The new compound was shown to have a slight positive effect on in vitro mineralization of SaOS-2 human osteosarcoma cells. However, it showed a significant increase of mineralized area at 20µg/mL, while at lower concentrations the effect was not of significant value. It should be noted however that an increase of the number of mineralized spots (nodules) at all concentrations tested was observed. This can be taken as an evidence for increased cell maturation and osteoblasticity. This data give rise to the notion that the compound has a contribution to the significant effect exhibited by the Feijoa sellowiana crude extract. References: 1. Hussein, S. A. M., Nawwar, M. A. M. (2006) Pharmazie 61: 2993-2996 2- Hussein, S. A. M., Nawwar, M. A. M.(2007). Phytochemistry 68: 1464-1470 Extra authors: Michael Linscheid, Vice President for research, Humboldt Universität zu Berlin, 12489 Berlin, Germany Email: michael.linscheid@cms.hu-berlin.de Manuela Harms, Institut für Pharmazie, Pharmazeutische Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Germany Email: Harms@uni-greifswald.de Kristian Wende, Institut für Pharmazie, Pharmazeutische Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Germany Email: wende@uni-greifswald.de Ulrike Lindequist Institut für Pharmazie, Pharmazeutische Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Germany Email: lindequi@uni-greifswald.de Presenter: Mahmoud A. M. Nawwar Department of Phytochemistry, Division of Pharmaceutical Industries, National Research Center, Dokki, Cairo, Egypt