The ANTIPLASMODIAL AND Β-HEMATIN INHIBITORY POTENTIAL OF THE EXTRACT AND SOLVENT FRACTIONS OF NEPHROLEPIS UNDULATA

Authors

  • Oluwayomi Rebecca AKOSILE Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife
  • Samuel Akintunde Odediran Obafemi Awolowo University, Ile-Ife https://orcid.org/0000-0002-8377-7899
  • Rachael Adebola BAMIGBOYE Natural History Museum, Obafemi Awolowo University, Ile Ife
  • Praise Adetola ADEBAYO Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife
  • Deborah Abimbola ADEBOLU Department of Pharamcognosy, obafemi Awolowo University, Ile Ife https://orcid.org/0009-0008-6363-6264

DOI:

https://doi.org/10.21010/Ajtcamv20n1.2

Keywords:

Antimalarial, Beta hematin inhibition; Nephrolepis undulata, Plasmodium berghei berghei, partitioned fractions.

Abstract

Background: The beta-hematin-inhibitory potential of the extract and the partitioned fractions of Nephrolepis undulata have not been determined, neither the likely constituents responsible for the observed in vivo antiplasmodial activities identified, hence this study.   

Materials and Methods: The methanol extract of N. undulata (MNU), was evaporated in vacuo and subsequently partitioned to give HNU (n hexane), DNU (dichloromethane), ENU (ethyl acetate) and ANU (aqueous phase)  which were investigated  in vivo in mice at 0-800mg/kg against chloroquine-sensitive Plasmodium berghei berghei using  Peter’s four-day test.  The Beta hematin inhibition assay was carried out on the extract and the fractions as well as Gas Chromatography Mass Spectroscopy for identification of the chemical constituents. Normal saline and chloroquine (10mg/kg) were negative and positive controls, respectively.  All the results were subjected to statistical analysis using ANOVA with Student Newman Keul’s as post hoc test.

Results: The partitioned fractions elicited comparatively lower percentages chemosuppression/inhibition and higher effective doses/inhibitory IC50 compared to NU in both experiments respectively suggesting that the constituents were acting synergistically. The GC-MS analysis revealed a total of eleven compounds in MNU partitioned into HNU: Neophytadiene, Hexadecanoic acid methyl ester, N--Hexadecanoic acid, 10 octa decenoic acid methyl ester and Methyl stearate, DNU: 2 ethyl acridine, ENU: Benzoic acid methyl ester and ANU: Benzoic acid methyl ester, 2 ethyl acridine while additional five compounds found in MNU were not detected in any of the fractions.

Conclusion: The constituents of the antimalarial MNU seem to be acting synergistically to inhibit haemozoin formation in the parasite.

Author Biographies

Samuel Akintunde Odediran, Obafemi Awolowo University, Ile-Ife

Deparment of Pharmacognosy, Obafemi Awolowo University, Ile Ife; Reader

Rachael Adebola BAMIGBOYE, Natural History Museum, Obafemi Awolowo University, Ile Ife

Natural History Museum, Obafemi Awolowo University, Ile Ife; Senior Lecturer

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Published

2024-12-20

How to Cite

AKOSILE, O. R., Odediran, S. A., BAMIGBOYE, R. A., ADEBAYO, P. A., & ADEBOLU, D. A. (2024). The ANTIPLASMODIAL AND Β-HEMATIN INHIBITORY POTENTIAL OF THE EXTRACT AND SOLVENT FRACTIONS OF NEPHROLEPIS UNDULATA. African Journal of Traditional, Complementary and Alternative Medicines, 20(1), 8–20. https://doi.org/10.21010/Ajtcamv20n1.2

Issue

Vol. 20 No. 1 (2023)

Section

Research Papers

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Copyright (c) 2024 Oluwayomi Rebecca AKOSILE, Samuel Akintunde Odediran, Rachael Adebola BAMIGBOYE, Praise Adetola ADEBAYO, Deborah Abimbola ADEBOLU

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