ISOLATION AND CHARACTERIZATION OF ANTINEOPLASTIC ALKALOIDS FROM CATHARANTHUS ROSEUS L. DON. CULTIVATED IN EGYPT

Authors

  • K. A. Shams
  • N. M. Nazif
  • N. S. Abdel Azim
  • K. A. Abdel Shafeek
  • M. L. El-Missiry
  • S. I. Ismail
  • M. M. Seif El Nasr

Keywords:

Catharanthus roseus L., Apocyanaceae, Vinblastine, Vincrisitine, Antileuckemic alkaloids, VLC, HPLC, HPTLC.

Abstract

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).

Author Biography

K. A. Shams

Phytochemistry Department, National Research Centre, El bohooth St., Dokki, Giza, Egypt, 12311.

Downloads

Published

2009-03-07

How to Cite

Shams, K. A., Nazif, N. M., Abdel Azim, N. S., Abdel Shafeek, K. A., El-Missiry, M. L., Ismail, S. I., & Seif El Nasr, M. M. (2009). ISOLATION AND CHARACTERIZATION OF ANTINEOPLASTIC ALKALOIDS FROM CATHARANTHUS ROSEUS L. DON. CULTIVATED IN EGYPT. African Journal of Traditional, Complementary and Alternative Medicines, 6(2), 118–122. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/568

Issue

Vol. 6 No. 2 (2009)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.