Vol 16 No 2 (2019)
Research Papers

ANTIMICROBIAL AND ANTIRADICAL PROPERTIES OF HAMMADA SCOPARIA (POMEL) ILJIN

Aziz Drioiche
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Nadia Benhlima
Research team on the chemistry of bioactive molecules and environment Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Samira Kharchouf
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Fadoua EL MAKHOUKHI
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Smahane MEHANNED
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Imad ADADI
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Hicham AAZIZ
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Ferdinand Kouoh Elombo
Laboratory of Pharmacology, Pharmacokinetics, and Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, Lille, France
Bernard Gressier
Laboratory of Pharmacology, Pharmacokinetics, and Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, Lille, France
Bruno ETO
65, Avenue de l’Hippodrome 62000 Arras, France. E-mail: etobr@laboratoires-tbc.com
Touriya Zair
Moulay Ismaïl University, Faculty of Sciences, Meknes, B.P. 11201 Zitoune Meknes, Morocco
Published January 9, 2020
Keywords
  • polyphenolics, Hammada scoparia, antimicrobial, antifungal, antioxidant
How to Cite
Drioiche, A., Benhlima, N., Kharchouf, S., EL MAKHOUKHI, F., MEHANNED, S., ADADI, I., AAZIZ, H., Kouoh Elombo, F., Gressier, B., ETO, B., & Zair, T. (2020). ANTIMICROBIAL AND ANTIRADICAL PROPERTIES OF HAMMADA SCOPARIA (POMEL) ILJIN. African Journal of Traditional, Complementary and Alternative Medicines, 16(2), 1-14. https://doi.org/10.21010/Ajtcam.v16n2.1

Abstract

Background: Hammada scoparia (Pomel) Iljin (HS), is commonly used by traditional healers in Morocco against microbial and fungal infections. We studied antimicrobial, antifungal and antiradical effects of organic extracts in vitro in order to confirm traditional utilization after phytochemical screening.

Materials and methods: Aerial parts of HS have been extracted by hydro-distillation using Clevenger-type apparatus, and the chemical composition was realized by Gas Chromatography coupled with Mass Spectroscopy (GC/MS). The antioxidant activity has been evaluated using DPPH test, while the antimicrobial tests of HS extract were conducted on twenty-eight bacterial strains and antifungal on twelve fungal strains.

Results: Chemical characterization of HS essential oils (EO) confirmed the presence of carvacrol (82,28%), p-cymene (2,52%), γ-terpinene (2,18%) and Z-caryophyllene (2,04%). Antimicrobial tests of HS extract showed a moderate antibacterial activity without antifungal effect. In addition, HS exhibited a very powerful antiradical activity with IC50 = 1,2 mg/ml compared to that of ascorbic acid (IC50 = 0,5 mg/mL) and butylated hydroxyanisole (0,8 mg/mL).

Conclusion: To our knowledge, this is the first demonstration that HS directly inhibits the growth of microorganisms in vitro, and further validates its traditional use as an antiseptic by traditional Moroccan healers.