RETRACTION: ANANAS SATIVA INCREASES LONGEVITY THROUGH OXIDATIVE STRESS RESISTANCE IN DROSOPHILA MELANOGASTER

Authors

  • M.O Ajagun-Ogunleye Department of Biochemistry, Kampala International University-Western Campus Uganda.

DOI:

https://doi.org/10.21010/ajtcamv15i3.9

Keywords:

Longevity, Ananas sativa fractions, antioxidants, free radical scavenging, oxidative stress resistance, Drosophila melanogaster.

Abstract

Background: Ageing is the major risk factor for most non-communicable diseases. It occurs as a result of free radical damage to macromolecules in the cell, coupled with environmental stress assaults. In the quest to identify new anti-ageing bioactive compounds from natural products, and despite the therapeutic values of Ananas sativa, its anti-ageing activity has not been fully elucidated. Therefore, the aim of this study was to screen the crude, methanol fruit extract of Ananas sativa (MEAS) and its fractions, for anti-ageing bioactivity in the fruit fly, Drosophila melanogaster w1118 wild type flies. Materials and Methods: Flies reared on food supplemented with 5, 10, and 20mg/ml fruit pulp and juice extract were assayed for longevity, fertility and stress resistance according to established protocols. Phytochemical composition and scavenging activity of extract on 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) were equally evaluated. The crude extract anti-ageing activity was assayed through a successive bioassay-guided fractionation approach, with different extraction solvents; n-Hexane, Chloroform, Ethyl acetate and Acetone. Results: There was a dose-dependent effect of the fruit extract on lifespan, fertility and oxidative stress resistance, with a unique information on the anti-ageing bioactivity of Ananas sativa. The IC50 values of the fruit extract and Ascorbic acid positive control in the free radical scavenging activity were 248.15μg/ml and 81.51μg/ml respectively. However, the bioactive fraction obtained from the Ethylacetate fraction F1, did not exhibit oxidative stress resistant effect in the model organism. Conclusion: The methanol fruit extract of Ananas sativa possesses anti-ageing bioactivity through oxidative stress resistance.

Author Biography

M.O Ajagun-Ogunleye, Department of Biochemistry, Kampala International University-Western Campus Uganda.

Department of Biochemistry, Kampala International University-Western Campus Uganda.

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Published

2018-05-30

How to Cite

Ajagun-Ogunleye, M. (2018). RETRACTION: ANANAS SATIVA INCREASES LONGEVITY THROUGH OXIDATIVE STRESS RESISTANCE IN DROSOPHILA MELANOGASTER. African Journal of Traditional, Complementary and Alternative Medicines, 15(3), 72–83. https://doi.org/10.21010/ajtcamv15i3.9

Issue

Section

Research Papers