MODULATORY EFFECT OF PERIOSTRACUM CICADAE AND BETULAE CORTEX EXTRACTS ON THE ACTIVATION OF ATOPIC DERMATITIS-RELATED ION CHANNELS ORAI1 AND TRPV3

Authors

  • Joo Hyun Nam Department of Physiology, College of Medicine, and Channelopathy Research Center (CRC), Dongguk University, 123 Dongdare-ro, Gyeongju 38066, Republic of Korea.
  • Hyo Won Jung
  • Woo Kyung Kim
  • Hyo Sang Bae

DOI:

https://doi.org/10.21010/ajtcam.vi15.1.19

Keywords:

Cryptotympana pustulata, Betula platyphylla var. japonica, calcium channels, modulatory effect

Abstract

Background: The cast-off shells of Cryptotympana pustulata (Periostracum Cicadae, PC) and the bark of Betula platyphylla (Betulae Cortex, BC) are used as traditional medicines for the treatment of skin diseases. This study was conducted to investigate the regulatory effects of PC and BC extracts on the activation of the ion channels, calcium release-activated calcium channel protein 1 (ORAI1) and transient receptor potential cation channel subfamily V member 3 (TRPV3). Materials and Methods: Human HEK293T cells, co-overexpressing ORAI1/stromal interaction molecule 1 (STIM1) or overexpressing TRPV3, were treated with PC or BC extracts at 0.1 mg/mL. The changes in ORAI1 and TRPV3 activities were measured using a conventional whole-cell patch-clamp technique. Results: PC and BC extracts significantly decreased ORAI1 activation in ORAI1-STIM1 co-overexpressing HEK293T cells and significantly increased TRPV3 activation in TRPV3 overexpressing cells, compared to that of 2- aminoethoxydiphenyl borate (2-APB, 100 μM), a known agonist of TRPV3. Conclusion: Our results suggest that PC and BC extracts have therapeutic potential to improve skin barrier abnormalities in atopic dermatitis via modulation of ORAI1 and TRPV3 activation.

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Published

2017-12-29

How to Cite

Nam, J. H., Jung, H. W., Kim, W. K., & Bae, H. S. (2017). MODULATORY EFFECT OF PERIOSTRACUM CICADAE AND BETULAE CORTEX EXTRACTS ON THE ACTIVATION OF ATOPIC DERMATITIS-RELATED ION CHANNELS ORAI1 AND TRPV3. African Journal of Traditional, Complementary and Alternative Medicines, 15(1), 183–187. https://doi.org/10.21010/ajtcam.vi15.1.19

Issue

Vol. 15 No. 1 (2018)

Section

Short communications

License

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