SIMULTANEOUS DETERMINATION AND ANTI-INFLAMMATORY EFFECTS OF TRADITIONAL HERBAL MEDICINE, MAHWANG-TANG

Authors

  • Chang-Seob Seo
  • Sae-Rom Yoo
  • Soo-Jin Jeong
  • Hyekyung Ha

DOI:

https://doi.org/10.21010/ajtcam.v14i5.20

Keywords:

Simultaneous determination, anti-Inflammatory effect, traditional herbal medicine, Mahwang-tang, HPLC–PDA

Abstract

Background: Mahwang-tang (MT) is a traditional Korean medicine consisting of six medicinal herbs and is used to treat the influenza-like diseases. Materials and Methods: We performed the simultaneous analysis of nine bioactive components in an MT sample using a high-performance liquid chromatography–diode array detector (HPLC–DAD) analysis. In addition, the MT sample was investigated the inhibitory effects against lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Results: Recovery of the nine marker compounds was 98.24–102.05% and relative standard deviations of intra-day and inter-day precisions of this method were 0.05–1.92% and 0.02–1.64%, respectively. Amounts of the nine bioactive compounds in the MT samples were 0.24–24.86 mg/g. To determine the biological activity of MT, its effects on the inflammatory reaction in LPS-treated RAW 264.7 macrophages were tested. The results suggest that MT possesses anti-inflammatory activity via suppression of the nitric oxide/prostaglandin E2 pathway. Conclusion: The established analytical method by HPLC–DAD is expected to help the quality control of MT samples or related herbal prescriptions. Our data also suggest that MT may be a potential therapeutic candidate for various inflammatory diseases.

Author Biographies

Chang-Seob Seo

K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Korea.

Sae-Rom Yoo

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon, 34054, Korea.

Soo-Jin Jeong

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon, 34054, Korea.

Hyekyung Ha

K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Korea.

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Published

2017-10-01

How to Cite

Seo, C.-S., Yoo, S.-R., Jeong, S.-J., & Ha, H. (2017). SIMULTANEOUS DETERMINATION AND ANTI-INFLAMMATORY EFFECTS OF TRADITIONAL HERBAL MEDICINE, MAHWANG-TANG. African Journal of Traditional, Complementary and Alternative Medicines, 14(5), 166–180. https://doi.org/10.21010/ajtcam.v14i5.20

Issue

Vol. 14 No. 5 (2017)

Section

Research Papers

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