SIMULTANEOUS DETERMINATION AND ANTI-INFLAMMATORY EFFECTS OF TRADITIONAL HERBAL MEDICINE, MAHWANG-TANG
Keywords:Simultaneous determination, anti-Inflammatory effect, traditional herbal medicine, Mahwang-tang, HPLC–PDA
AbstractBackground: Mahwang-tang (MT) is a traditional Korean medicine consisting of six medicinal herbs and is used to treat the influenza-like diseases. Materials and Methods: We performed the simultaneous analysis of nine bioactive components in an MT sample using a high-performance liquid chromatography–diode array detector (HPLC–DAD) analysis. In addition, the MT sample was investigated the inhibitory effects against lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Results: Recovery of the nine marker compounds was 98.24–102.05% and relative standard deviations of intra-day and inter-day precisions of this method were 0.05–1.92% and 0.02–1.64%, respectively. Amounts of the nine bioactive compounds in the MT samples were 0.24–24.86 mg/g. To determine the biological activity of MT, its effects on the inflammatory reaction in LPS-treated RAW 264.7 macrophages were tested. The results suggest that MT possesses anti-inflammatory activity via suppression of the nitric oxide/prostaglandin E2 pathway. Conclusion: The established analytical method by HPLC–DAD is expected to help the quality control of MT samples or related herbal prescriptions. Our data also suggest that MT may be a potential therapeutic candidate for various inflammatory diseases.
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