HERB-RESISTANT UPEC STRAINS HAVE DIFFERENT BIOFILM FORMATION ABILITIES AND DIFFERENT EXPRESSION OF CERTAIN BIOFILM RELATED GENES

Authors

  • YanQing Tong
  • LiLi Dou
  • Ye Sun
  • DongKai Zhao

DOI:

https://doi.org/10.21010/ajtcam.v14i5.8

Keywords:

Herb-resistance, Biofilm formation, Uropathogenic Escherichia coli (UPEC), pga ABCD, luxS.

Abstract

Background: The mechanisms and patterns of drug resistances of E. coli strains that cause uncomplicated urinary tract infections (UTIs) vary considerably. The emerging herbresistance of uropathogenic Escherichia coli (UPEC) has been a serious health problem, yet with unknown underlying mechanisms. Methods: To explore the potential herb-resistance mechanisms of E. coli strains that cause uncomplicated UTIs, three clinically isolated herb-resistant UPEC strains (1351, 4996, 5028) were analyzed for their abilities to form biofilms and the expressions of the pga ABCD and luxS genes. Results: We found that the expression of pgaA and pgaB are very different between 1351 and 5028, and the loss-offunction of luxS in 4996 has impact on biofilm formation. Conclusions: 1. Herb-resistance of the strains is related to their abilities of biofilm formation. 2. Biofilm formation capabilities of herb-resistant strains show different responses to the presence of glucose. 3. luxS encoded AI-2 is not essential for biofilm formation in this strain but may help with more biofilm formation.

Author Biographies

YanQing Tong

Department of Nephrology, The Affilliated Hospital to Changchun University of Chinese Medicine, Changchun, China;

LiLi Dou

Department of Nephrology, The Affilliated Hospital to Changchun University of Chinese Medicine, Changchun, China;

Ye Sun

Department of Microbiology, The Affilliated Hospital to Changchun University of Chinese Medicine, Changchun, China.

DongKai Zhao

Department of Microbiology, The Affilliated Hospital to Changchun University of Chinese Medicine, Changchun, China.

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Published

2017-10-01

How to Cite

Tong, Y., Dou, L., Sun, Y., & Zhao, D. (2017). HERB-RESISTANT UPEC STRAINS HAVE DIFFERENT BIOFILM FORMATION ABILITIES AND DIFFERENT EXPRESSION OF CERTAIN BIOFILM RELATED GENES. African Journal of Traditional, Complementary and Alternative Medicines, 14(5), 56–62. https://doi.org/10.21010/ajtcam.v14i5.8

Issue

Vol. 14 No. 5 (2017)

Section

Research Papers

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