CHEMICAL COMPOSITION AND PROPHYLACTIC EFFECTS OF SATURJA KHUZESTANICA ESSENTIAL OIL ON ACUTE TOXOPLASMOSIS IN MICE
Keywords:GC/MS, Satureja khuzestanica, Toxoplasma gondii, In vivo, Mice
AbstractBackground: Toxoplasma gondii is a widespread zoonotic protozoan that infects approximately one third of the global human population and all other warm-blooded animals. The present study aims to evaluate the prophylactic effects of Satureja khuzestanica essential oil (SKEO) on infected mice with acute toxoplasmosis. Materials and Methods: The components of the SKEO were identified by gas chromatography/mass spectroscopy (GC/MS). To evaluate the prophylactic effects of SKEO, mice were divided into four groups. (i) non-treated group, (ii) mice treated with olive oil once a day for two weeks, (iii) mice treated with SKEO at the dose of 0.2ml/kg once a day for two weeks, (iv) and mice orally treated with SKEO at the dose of 0.3 ml/kg once a day for two weeks. After 24 h (fifteenth day) mice in the groups of two-four were infected intraperitonealy with 10-4 tachyzoite of T. gondii, RH strain. The mortality rate in all infected mice and the number of tachyzoites from infected mice were recorded. Results: The main components of SKEO were carvacrol (78.8%), thymol (7.5%), and beta-Bisabolene (1.2%). Findings of prophylactic effects revealed that mortality rate of infected mice was 8 days after oral administration of SKEO at the concentration of 0.2 and 0.3ml/kg (P<0.05). In contrast, this value for control group was 5 days. In addition, SKEO significantly reduced the mean number of tachyzoites compared with control group (P<0.05). No significant difference (P>0.05) was observed in the clinical chemistry and hematological parameters following oral administrations of SKEO at the doses of 0.2 and 0.3 ml/kg for 14 days. Conclusion: The results showed the potential of SKEO as a natural source for the production of new prophylactic agent for use in toxoplasmosis.
How to Cite
Copyright: Creative Commons Attribution CC.
This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.