APOPTOSIS INDUCTION OF EPIFRIEDELINOL ON HUMAN CERVICAL CANCER CELL LINE

Authors

  • Jie Yang Women's Health Center, Women and Children's Hospital of LinYi, Linyi, Shandong, China, 276000.
  • Jing Fa
  • Bingxing Li

DOI:

https://doi.org/10.21010/ajtcam.v14i4.10

Keywords:

Epifriedelinol, cervical cancer, Apoptosis, MTT assay, C33A and HeLa

Abstract

Background: Present investigation evaluates the antitumor activity of epifriedelinol for the management of cervical cancer by inducing process of apoptosis. Methods: Human Cervical Cancer Cell Line, C33A and HeLa were selected for study and treated with epifriedelinol at a concentration of (50-1000 μg/ml). Cytotoxicity of epifriedelinol was estimated by MTT assay and induction of apoptosis was assessed by estimating the activity of caspase 3, 8 and 9 enzyme, apoptosis assay and translocation of cytochrome c. Moreover an expression of several proteins that plays role in the apoptosis process was estimated by western blot method. Results: Result of the study suggested that treatment with epifriedelinol significantly decrease the viability count of cancerous cell in a dose perndent manner and also enhances the formation of oligonucleosome in both the cell lines. However activity of caspase enzymes and translocation of cytochrome c were enhanced after treatment with epifriedelinol. It was also observed that epifriedelinol treatment alters the ratio of pro-apoptotic to anti-apoptotic proteins and enhances the expressions of inhibitor of apoptosis proteins (IAP). Conclusion: Result of our study proves the anticancer activity of epifriedelinol in cervical cancer by inducing apoptosis as treatment with it enhances the production of oligonucleosomes, translocation of cytochrome c and activity caspase enzymes.

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Published

2017-06-05

How to Cite

Yang, J., Fa, J., & Li, B. (2017). APOPTOSIS INDUCTION OF EPIFRIEDELINOL ON HUMAN CERVICAL CANCER CELL LINE. African Journal of Traditional, Complementary and Alternative Medicines, 14(4), 80–86. https://doi.org/10.21010/ajtcam.v14i4.10

Issue

Vol. 14 No. 4 (2017)

Section

Research Papers

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