KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

Authors

  • Saheed Sabiu Phytomedicine, Food Factors and Toxicology Research Laboratory, Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete
  • Emmanuel Oladipo Ajani Plant and Environmental Biochemistry Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
  • Taofik Olatunde Sunmonu Phytomedicine and Phytopharmacology Research Group, Plant Sciences Department, University of the Free State, Qwaqwa campus, Phuthaditjhaba 9866, South Africa
  • Anofi Omotayo Tom Ashafa Phytomedicine and Phytopharmacology Research Group, Plant Sciences Department, University of the Free State, Qwaqwa campus, Phuthaditjhaba 9866, South Africa

DOI:

https://doi.org/10.21010/ajtcam.v14i4.6

Keywords:

Acarbose, Antioxidant, Diabetes, Enzyme kinetics, Vmax

Abstract

Background: The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. Materials and Methods: This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro. The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. Results: The data obtained revealed that the extract moderately and potently inhibited the specific activities of α-amylase and α-glucosidase, respectively. The inhibition was concentration-related with respective IC50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. Conclusion: The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α-amylase and α-glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.

Downloads

Published

2017-06-05

How to Cite

Sabiu, S., Ajani, E. O., Sunmonu, T. O., & Ashafa, A. O. T. (2017). KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES. African Journal of Traditional, Complementary and Alternative Medicines, 14(4), 46–53. https://doi.org/10.21010/ajtcam.v14i4.6

Issue

Vol. 14 No. 4 (2017)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.