March 7, 2009
- Dalbergia sissoo,
- Biomphalaria pfeifferi,
- Schistosoma mansoni,
- Egg masses
How to Cite
Adenusi, A., & Odaibo, A. (2009). EFFECTS OF VARYING CONCENTRATIONS OF THE CRUDE AQUEOUS AND ETHANOLIC. African Journal of Traditional, Complementary and Alternative Medicines, 6(2), 139-149. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/465
This study evaluated, using replicated laboratory bioassays, the toxicities of the crude aqueous and
ethanolic extracts of Dalbergia sissoo Roxb. 1832 (family Leguminosae) fruits, leaves, roots and stem bark
against egg masses of Biomphalaria pfeifferi (Krauss, 1848), the snail intermediate host of Schistosoma mansoni
(Sambon, 1907) in Nigeria. Viable 0-24 hr-old embryonated egg masses were separately exposed to five
different concentrations (7.81-2000 mg/l) of extracts for 24 hrs, washed in dechlorinated tap water and incubated
at room temperature for a maximum of 4 weeks. The LC50 and LC90 values of test extracts for egg masses were
calculated by probit analysis. The activities of the tested extracts were concentration-dependent. However, only
the ethanolic extract of the fruits demonstrated significant activity (24 hr-LC90 value < 100 mg/l: 89.29 mg/l).
Mortalities of eggs were manifested at the gastrula/exogastrula and or the prehatch snail stage of development.
The percentage of dead embryos at the prehatch snail stage decreased while the deaths of embryos at the
gastrula/exogastrula stage increased, with increasing concentration of extract. Lethality of the ethanolic extract
of D. sissoo fruits to embryonated egg masses of B. pfeifferi is an added advantage to its potential development
for use as a plant molluscicide, as the overall efficacy of a molluscicide is greatly enhanced if it also shows
significant toxicity towards snail eggs.