Vol 6 No 2 (2009)
Research Papers


Adedotun Adesegun Adenusi
Olabisi Onabanjo University, Ago-Iwoye.
Alexander Bababunmi Odaibo
University of Ibadan, Ibadan.
Published March 7, 2009
  • Dalbergia sissoo,
  • Biomphalaria pfeifferi,
  • Schistosoma mansoni,
  • Egg masses
How to Cite
Adenusi, A., & Odaibo, A. (2009). EFFECTS OF VARYING CONCENTRATIONS OF THE CRUDE AQUEOUS AND ETHANOLIC. African Journal of Traditional, Complementary and Alternative Medicines, 6(2), 139-149. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/465


This study evaluated, using replicated laboratory bioassays, the toxicities of the crude aqueous and ethanolic extracts of Dalbergia sissoo Roxb. 1832 (family Leguminosae) fruits, leaves, roots and stem bark against egg masses of Biomphalaria pfeifferi (Krauss, 1848), the snail intermediate host of Schistosoma mansoni (Sambon, 1907) in Nigeria. Viable 0-24 hr-old embryonated egg masses were separately exposed to five different concentrations (7.81-2000 mg/l) of extracts for 24 hrs, washed in dechlorinated tap water and incubated at room temperature for a maximum of 4 weeks. The LC50 and LC90 values of test extracts for egg masses were calculated by probit analysis. The activities of the tested extracts were concentration-dependent. However, only the ethanolic extract of the fruits demonstrated significant activity (24 hr-LC90 value < 100 mg/l: 89.29 mg/l). Mortalities of eggs were manifested at the gastrula/exogastrula and or the prehatch snail stage of development. The percentage of dead embryos at the prehatch snail stage decreased while the deaths of embryos at the gastrula/exogastrula stage increased, with increasing concentration of extract. Lethality of the ethanolic extract of D. sissoo fruits to embryonated egg masses of B. pfeifferi is an added advantage to its potential development for use as a plant molluscicide, as the overall efficacy of a molluscicide is greatly enhanced if it also shows significant toxicity towards snail eggs.