EFFECT OF TRIPTOLIDE ON PROLIFERATION AND APOPTOSIS OF ANGIOTENSIN II-INDUCED CARDIAC FIBROBLASTS IN VITRO: A PRELIMINARY STUDY

Authors

  • Mao Liu
  • James Yeh
  • Yin Huang
  • Alfredo Redondo
  • Jianting Ke
  • Jierong Yao
  • Guangyi Tan
  • Wenyi Tang
  • Jian Chen

DOI:

https://doi.org/10.21010/ajtcam.v14i1.16

Keywords:

Cardiac fibroblast, triptolide, proliferation, apoptosis, angiotensin

Abstract

Background: The effect of triptolide (TPL) on cardiac fibroblasts (CFbs) and cardiac fibrosis remain unknown till now. This study was conducted to explore the effects of TPL on proliferation and apoptosis of angiotensin II (Ang II)-induced CFbs. Materials and Methods: Ang II was used to promote proliferation of CFbs. Two dosages of TPL (10ng/ml and 100ng/ml) were chosen. MTT assay was used to detect cell survival rate in vitro. Flow cytometer was performed to analyze apoptosis of CFbs. Hydroxyproline concentration was detected with hydroxyproline assay kit. Quantitative real-time PCR was used to detect the expression of TGF-

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Published

2016-11-23

How to Cite

Liu, M., Yeh, J., Huang, Y., Redondo, A., Ke, J., Yao, J., Tan, G., Tang, W., & Chen, J. (2016). EFFECT OF TRIPTOLIDE ON PROLIFERATION AND APOPTOSIS OF ANGIOTENSIN II-INDUCED CARDIAC FIBROBLASTS IN VITRO: A PRELIMINARY STUDY. African Journal of Traditional, Complementary and Alternative Medicines, 14(1), 145–154. https://doi.org/10.21010/ajtcam.v14i1.16

Issue

Vol. 14 No. 1 (2017)

Section

Research Papers

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