INFLUENCE OF MITRAGYNA CILIATA (MYTA) ON THE MICROSOMAL ACTIVITY of ATPase Na+/K+ DEPENDENT EXTRACT ON A RABBIT HEART

Authors

  • Alain dit Philippe Bidie
  • Ernest Koffi
  • Jean-David N’Guessan
  • Joseph Allico Djaman
  • Frédérique Guede-Guina

Keywords:

Mitragyna ciliata, ATPase Na /K , inhibitors of ATPase Na /K .

Abstract

Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat’s isolated heart. In this work we studied the effect of MYTA on microsomal Na+/K+ -dependant ATPase (Na+, K+ ATPase) extracted from the heart of a rabbit since digitalics inhibit Na+, K+ ATPase. Our results revealed that the Na+/K+ ATPase has an optimum pH of 7.4 and temperature of 37oC respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 µM/min. LINEWEAVER and BURK plots showed that MYTA did not alter KM (1.31 mM) but decreased VMAX. This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na+/K+ ATPase extracted from rabbit heart with a Ci50 of 48 µg / ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na+/K+ ATPase like cardiotonics of the digitalic type.

Author Biographies

Alain dit Philippe Bidie

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Ernest Koffi

Laboratoire de Biochimie et de Sciences des Aliments ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire.

Jean-David N’Guessan

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Joseph Allico Djaman

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Frédérique Guede-Guina

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Downloads

Published

2008-04-08

How to Cite

Bidie, A. dit P., Koffi, E., N’Guessan, J.-D., Djaman, J. A., & Guede-Guina, F. (2008). INFLUENCE OF MITRAGYNA CILIATA (MYTA) ON THE MICROSOMAL ACTIVITY of ATPase Na+/K+ DEPENDENT EXTRACT ON A RABBIT HEART. African Journal of Traditional, Complementary and Alternative Medicines, 5(3), 294–301. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/423

Issue

Vol. 5 No. 3 (2008)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.