ROLE OF WORMWOOD (ARTEMISIA ABSINTHIUM) EXTRACT ON OXIDATIVE STRESS IN AMELIORATING LEAD INDUCED HAEMATOTOXICITY

Authors

  • omar kharoubi
  • M. Slimani
  • D. Krouf
  • L. Seddik
  • A. Aoues

Keywords:

Antioxidant defence system - Lead acetate - Lipid peroxidation - Red Blood Cell – Rat - Wormwood (Artemisia absinthium) plant extract.

Abstract

Effects of ROS generation have been postulated to be major contributors to lead-exposure related disease. The aim of the study was to investigate the effect of aqueous extract of wormwood (Artemisia absinthium) on oxidative stress in rats protractedly exposed to lead. Aqueous extract of wormwood plant was administered orally (200 mg.kg-1 body weight). Plasma vitamin C, E and non-protein thiol concentrations, red blood cells (RBC) thiobarbituric acid reactive substances, reduced glutathione levels and haemolysis test were evaluated. In addition, RBC antioxidant enzymes activities such as superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase were also estimated. After 11-weeks, significant decreases of plasma vitamin C, E, non protein-thiol (NP-SH) and RBC-reduced glutathione levels were observed in Pb compared to control group (-32.9%, -57.1%, -53.1%, -33.9%, respectively); superoxide dismutase, glutathione peroxidase, uric aminolevulinic acid and haemolysis test significantly increased in Pb compared to control group (+64.3%, +40.3%, +145%, +44.3%, respectively). In our investigation, after 4-weeks of treatment all treated groups did not show any difference compared to the control group, except for glutathione peroxidase and RBC-superoxide dismutase activity (-15.7% and +16.4%, respectively). The findings of this study suggest that wormwood (Artemisia absinthium) extract restored the enzymes activities perturbed by exposure to lead, and had a protective role against lipid peroxidation

Author Biographies

omar kharoubi

Department of Biology, Faculty of Science; laboratory of biochemistry. University of Es-senia Oran, Algeria

M. Slimani

Department of Biology, Faculty of Science; laboratory of biochemistry. University of Es-senia Oran, Algeria

D. Krouf

Department of Biology, Faculty of Science; laboratory of Clinical and Metabolic Nutrition. University of Es-senia Oran, Algeria

L. Seddik

Department of Biology, Faculty of Science; laboratory of biochemistry. University of Es-senia Oran, Algeria

A. Aoues

Department of Biology, Faculty of Science; laboratory of biochemistry. University of Es-senia Oran, Algeria

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Published

2008-04-08

How to Cite

kharoubi, omar, Slimani, M., Krouf, D., Seddik, L., & Aoues, A. (2008). ROLE OF WORMWOOD (ARTEMISIA ABSINTHIUM) EXTRACT ON OXIDATIVE STRESS IN AMELIORATING LEAD INDUCED HAEMATOTOXICITY. African Journal of Traditional, Complementary and Alternative Medicines, 5(3), 263–270. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/337

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Section

Research Papers