IMPROVING THE SURVIVAL OF PROBIOTIC IN SIMULATED CONDITIONS AND AZOXYMETHANE-INDUCED COLON TUMOUR BEARING MICE USING MODIFIED CITRUS PECTIN-ALGINATE MICROENCAPSULATION

Authors

  • Frederick Odun-Ayo Department of Biotechnology and Food Technology Durban University of Technology
  • John Mellem
  • Lalini Reddy

DOI:

https://doi.org/10.21010/ajtcam.v13i2.13

Keywords:

Modified citrus pectin, alginate, probiotic, and microencapsulation.

Abstract

Background: For a probiotic to be viable it needs to be preserved at a recommended minimum level of 6–7 log10cfu/g in the product being consumed, as suggested by the International Dairy Federation. Different biopolymer matrices have been used for encapsulation of probiotic; however, loss of viability is still a challenge. Materials and Methods: Modified citrus pectin-alginate microbeads containing Lactobacillus acidophilus ATCC 4356 was developed. Efficiency of the microbeads was evaluated in simulated conditions of the gastrointestinal tract and in Balb/c mice induced with colon tumor. Genomic identification of faecal lactobacilli samples from treated mice was also performed. Results: The Modified citrus pectin-alginate probiotic microbeads significantly enhanced the viability of Lactobacillus acidophilus ATCC 4356 compared to the control (p< 0.05) both in vitro and in vivo. Exposure of the modified citrus pectin-alginate microbeads to 3 hours of simulated gastric juice resulted in 82.7% survival of L. acidophilus ATCC 4356. Also, the number of faecal lactobacilli in the modified citrus pectin-alginate probiotic treated mice increased by 10.2% after 28 days. Conclusion: Modified citrus pectin-alginate is a novel effective means of oral delivery of bacterial cells and bioactive compounds. Modified citrus pectin-alginate can be used in probiotic therapy which may improve the prevention of colon cancer.

Author Biography

Frederick Odun-Ayo, Department of Biotechnology and Food Technology Durban University of Technology

Department of Biotechnology and Food Technology - Senior Lecturer

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Published

2016-02-05

How to Cite

Odun-Ayo, F., Mellem, J., & Reddy, L. (2016). IMPROVING THE SURVIVAL OF PROBIOTIC IN SIMULATED CONDITIONS AND AZOXYMETHANE-INDUCED COLON TUMOUR BEARING MICE USING MODIFIED CITRUS PECTIN-ALGINATE MICROENCAPSULATION. African Journal of Traditional, Complementary and Alternative Medicines, 13(2), 101–109. https://doi.org/10.21010/ajtcam.v13i2.13

Issue

Vol. 13 No. 2 (2016)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

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