ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER ACTIVITIES OF STRYCHNOS LUCIDA R. BR.

Authors

  • Nevio da Costa Sarmento Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand
  • Apilak Worachartcheewan
  • Ratchanok Pingaew
  • Supaluk Prachayasittikul
  • Somsak Ruchirawat
  • Virapong Prachayasittikul

DOI:

https://doi.org/10.21010/ajtcam.v12i4.18

Keywords:

Strychnos lucida, antimicrobial activity, antioxidant activity, anticancer activity

Abstract

Backgroud: Strychnos lucida R. Br. (Loganiaceae), a well-known indigenous medicine in Timor Leste, has been used for the treatment of ailments such as malaria, diarrhoea, fever, hypertension, cancer, diabetes mellitus and skin infections. Its pharmacological activity has never been reported. The aim of this study was to determine the biological activities of S. lucida, including antimicrobial, antioxidant and anticancer activities. Materials and methods: The stem, stem bark, twig and leaves of S. lucida were extracted by non-polar (hexane) and polar (ethyl acetate and methanol) solvents. Antimicrobial activity of the plant extracts against 29 microorganisms was evaluated using the agar dilution method and antioxidant properties were determined using DPPH and SOD assays. Anticancer activity was investigated against HepG2, HuCCA-1, A549 and MOLT-3 cell lines using the MTT and XTT assays. Results: It was found that the hexane and ethyl acetate extracts of S. lucida selectively inhibited the growth of Gram positive bacteria (Bacillus subtilis ATCC 6633, Bacillus cereus and Streptococcus pyogenes) with MICs range of 32-128 g/mL. Antioxidant activities, radical and superoxide scavenging properties, were observed for ethyl acetate and methanol extracts of S. lucida. Particularly, the ethyl acetate extracts selectively showed inhibitory activity against MOLT-3 cells. Notably, the plant extracts showed the relationship between antimicrobial, antioxidant and anticancer activities. Conclusion: The findings support the medicinal usage of S. lucida.

Author Biography

Virapong Prachayasittikul

Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University

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Published

2015-06-27

How to Cite

Sarmento, N. da C., Worachartcheewan, A., Pingaew, R., Prachayasittikul, S., Ruchirawat, S., & Prachayasittikul, V. (2015). ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER ACTIVITIES OF STRYCHNOS LUCIDA R. BR. African Journal of Traditional, Complementary and Alternative Medicines, 12(4), 122–127. https://doi.org/10.21010/ajtcam.v12i4.18

Issue

Vol. 12 No. 4 (2015)

Section

Research Papers

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