THE PROTECTIVE EFFECT OF AN AQUEOUS EXTRACT FROM SMILAX EXCELSA L. AGAINST CARBON TETRACHLORIDE-INDUCED LIVER INJURY IN RATS

Authors

  • Nurten Ozsoy Department of Biochemistry, Faculty of Pharmacy, Istanbul University
  • Alper Okyar Department of Pharmacology, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey
  • Pelin Arda-Pirincci Department of Biology, Faculty of Science, Istanbul University, TR-34134 Istanbul-Turkey
  • Ayse Can Department of Biochemistry, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey
  • Sehnaz Bolkent Department of Biology, Faculty of Science, Istanbul University, TR-34134 Istanbul-Turkey
  • Nuriye Akev Department of Biochemistry, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey

DOI:

https://doi.org/10.21010/ajtcam.v13i3.24

Keywords:

Alternative Medicines

Abstract

Background: Because reactive oxygen species (ros) contribute to the pathogenesis of various acute and chronic liver diseases, dietary antioxidants and drugs from herbal origins have been proved to be beneficial as therapeutic agents in reversing hepatotoxicity and oxidative stress. The objective of this study was to investigate the protective effect of an aqueous extract from smilax excelsa l. Shoots and leaves against acute ccl4-induced liver injury as well as the changes in antioxidative defense system in female wistar albino rats. Materials and Methods: S. Excelsa extract was administered orally in doses of 100, 200 and 400 mg/kg body weight, once daily for 9 days. Acute hepatic toxicity was induced by intraperitoneal injection of ccl4 (1 ml/kg) on the 10th day. 24 h after ccl4 intoxication, biochemical and histopathological analyses were undertaken on sera and liver tissues. Results: Ccl4 challenge caused significant increases in the activities of liver enzymes as well as the levels of bilirubin, malondialdehyde and nitric oxide, while total serum protein levels and antioxidant defense system parameters were reduced significantly compared to the normal group. Administration of s. Excelsa extract at a dose of 400 mg/kg resulted in a suppression of ccl4-induced lipid peroxidation and altered oxidative stress parameters to nearly normal values in comparison to ccl4-treated rats. Nevertheless the extract did not reduce the extent of ccl4-induced mild liver injury, as seen by the histopathology of liver damage. Conclusion: The results of this study suggest that s. Excelsa could protect the liver tissues against ccl4-induced oxidative stress probably by increasing antioxidative defense activities.

Author Biographies

Nurten Ozsoy, Department of Biochemistry, Faculty of Pharmacy, Istanbul University

Department of Biochemistry Associated Professor

Alper Okyar, Department of Pharmacology, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey

Department of Pharmacology Associated Professor

Pelin Arda-Pirincci, Department of Biology, Faculty of Science, Istanbul University, TR-34134 Istanbul-Turkey

Department of Biology Associated Professor

Ayse Can, Department of Biochemistry, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey

Department of Biochemistry Professor

Sehnaz Bolkent, Department of Biology, Faculty of Science, Istanbul University, TR-34134 Istanbul-Turkey

Department of Biology Professor

Nuriye Akev, Department of Biochemistry, Faculty of Pharmacy, Istanbul University, TR-34116 Istanbul-Turkey

Department of Biochemistry Professor

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Published

2016-03-15

How to Cite

Ozsoy, N., Okyar, A., Arda-Pirincci, P., Can, A., Bolkent, S., & Akev, N. (2016). THE PROTECTIVE EFFECT OF AN AQUEOUS EXTRACT FROM SMILAX EXCELSA L. AGAINST CARBON TETRACHLORIDE-INDUCED LIVER INJURY IN RATS. African Journal of Traditional, Complementary and Alternative Medicines, 13(3), 203–208. https://doi.org/10.21010/ajtcam.v13i3.24

Issue

Section

Research Papers