ANTIOXIDANT ACTIVITY OF PIPER CANINUM AND CYCLOOXYGENASE-2 INHIBITION BY METHOXYLATED FLAVONES

Authors

  • Muhammad Helmi Nadri Department of Bioscience and Health Sciences, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
  • Mohammed Abu Naser Naser Department of Bioscience and Health Sciences, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
  • Razauden Mohamed Zulkifli Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
  • Ida Idayu Muhamad Bioprocess Engineering Department Faculty of Chemical Engineering Universiti Teknologi Malaysia 81310 UTM Skudai, Johor, Malaysia.
  • Farediah Ahmad Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
  • Wan Mohd Nuzul Hakimi Wan Salleh Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
  • Hasnah M. Sirat Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia

DOI:

https://doi.org/10.21010/ajtcam.v12i2.18

Keywords:

Piper caninum, antioxidant, docking, cyclooxygenase-2

Abstract

Background: The crude extracts and pure compounds isolated from Piper caninum was studied for their bioactivities. Material and method: The present study was carried out with the following aims: (1) to quantify the total phenolic content and free radical scavenging activities of the crude extracts by Folin-Ciocalteu and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. (2) to determine the cyclooxygenase-2 inhibition activities of selected isolated compounds as assessed by molecular docking and in vitro cyclooxygenase-2 inhibition assay. Results: Methanolic extracts of Piper caninum exhibited the highest total phenolic content and free radical scavenging activities. All the pure compounds possessed significant cyclooxygenase-2 inhibition at physiological concentrations. Conclusion: Based on our findings of the molecular docking and in vitro study, we therefore suggest that Piper caninum methoxylated flavones are potent inhibitors of cyclooxygenase-2 at physiological concentrations.

Author Biographies

Mohammed Abu Naser Naser, Department of Bioscience and Health Sciences, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia

Senior Lecturer

Ida Idayu Muhamad, Bioprocess Engineering Department Faculty of Chemical Engineering Universiti Teknologi Malaysia 81310 UTM Skudai, Johor, Malaysia.

Associate Professor

Farediah Ahmad, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia

Associate Professor

Hasnah M. Sirat, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia

Professor

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Published

2015-03-08

How to Cite

Nadri, M. H., Naser, M. A. N., Zulkifli, R. M., Muhamad, I. I., Ahmad, F., Wan Salleh, W. M. N. H., & Sirat, H. M. (2015). ANTIOXIDANT ACTIVITY OF PIPER CANINUM AND CYCLOOXYGENASE-2 INHIBITION BY METHOXYLATED FLAVONES. African Journal of Traditional, Complementary and Alternative Medicines, 12(2), 120–125. https://doi.org/10.21010/ajtcam.v12i2.18

Issue

Vol. 12 No. 2 (2015)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

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