EVALUATION OF ACUTE TOXICITY AND ANTI-INFLAMMATORY EFFECTS OF BACCHAROIDES SCHIMPERI (DC.) IN EXPERIMENTAL ANIMALS

Abstract

Background: Steroidal and non-steroidal anti-inflammatory drugs are most commonly used to treat inflammation, and shown to have severe side effects. In this study, we aimed at evaluating the anti-inflammatory and acute toxicity effects of Baccharoides schimperi (DC.) in order to get new anti-inflammatory agents of natural origin. Materials and methods: The aerial part of the plant was dried under shade, ground and extracted with 96% alcohol (BSE). It was further fractionated in sequence to n-hexane (BSH), chloroform (BSC) and methanol (BSM) soluble fractions. Acute toxicity was evaluated by oral administration of plant and hind paw induced-edema method in rats was used for the anti-inflammatory evaluation. Results: The BSE was found safe up to the dose level of 3 g/kg b.w. and showed LD50 value 7.250 g/kg body weight (b.w.) in mice. BSE showed significant anti-inflammatory effect (62.91%) at 500 mg/kg b.w. Further the n-hexane, chloroform and methanol fractions of BSE were tested for anti-inflammatory activity. The n-hexane fraction (BSH) exhibits significant activity (64.87%) at 400 mg/kg b.w. The methanol fraction (BSM) showed dose dependent activity, highest activity (60.42%) was observed at higher dose 400 of mg/kg b.w. In chloroform fraction (BSC) no significant activity was observed. Conclusion: The results of the study revealed that the plant is safe to the experimental model and recommended as a potential source of anti-inflammatory agent.