PROTECTIVE EFFECT OF MEXICAN PROPOLIS ON MDBK CELLS EXPOSED TO AUJESZKY'S DISEASE VIRUS (PSEUDORABIES VIRUS).

Authors

  • María de Jesús González Búrquez Universidad Nacional Autónoma de México
  • María de Lourdes Juárez Mosqueda Universidad Nacional Autónoma de México
  • Humberto Ramírez Mendoza Universidad Nacional Autónoma de México
  • Carlos Ignacio Soto Zárate Universidad Nacional Autónoma de México
  • Liborio Carrillo Miranda Universidad Nacional Autónoma de México
  • Tonatiuh Alejandro Crúz Sánchez Universidad Nacional Autónoma de México

DOI:

https://doi.org/10.21010/ajtcam.v12i4.16

Keywords:

Mexican propolis, PRV, MDBK cells, antiviral activity

Abstract

In this work, antiviral effect of Mexican propolis was evaluated in vitro on MDBK (Madin Darby Bovine Kidney) cell cultures infected with pseudorabies virus (PRV). First was carried the cytotoxicity test of the ethanol extract of propolis (EEP) on this cell culture, subsequently, the infective dose of virus Shope strain was determined for the infection of cell cultures and finally the interaction between EPP and virus was done at cell culture level (two hours before, during and two hours later of infection). Also, cell culture samples with the same treatments were processed for transmission electron microscopy (TEM) to observe possible alterations of propolis on the virus. It was determined that using the treatment of 0.5 mg / ml of EEP two hours before being infected, the culture shows a reduction in the number of plaque forming units compared to the other treatments or with the culture infected, it is important to note that the difference found was statistically significant (p <0.01). Moreover, structurally modified viral particles were observed by TEM, suggesting a possible damage to proteins of viral envelope causing a destabilization of the viral structure which could affect the penetration and replicative cycle of virus. This result opens research perspectives in order to explore the possibility of using propolis as a preventive method against pseudorabies disease.

Author Biographies

María de Jesús González Búrquez, Universidad Nacional Autónoma de México

Departament of Biological Sciences

María de Lourdes Juárez Mosqueda, Universidad Nacional Autónoma de México

Departament Morphology

Humberto Ramírez Mendoza, Universidad Nacional Autónoma de México

Departament of production swine

Carlos Ignacio Soto Zárate, Universidad Nacional Autónoma de México

Departament Biological Sciences

Liborio Carrillo Miranda, Universidad Nacional Autónoma de México

Departamen of Production Animal

Tonatiuh Alejandro Crúz Sánchez, Universidad Nacional Autónoma de México

Departament Biological Sciences

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Published

2015-06-27

How to Cite

Búrquez, M. de J. G., Mosqueda, M. de L. J., Mendoza, H. R., Zárate, C. I. S., Miranda, L. C., & Sánchez, T. A. C. (2015). PROTECTIVE EFFECT OF MEXICAN PROPOLIS ON MDBK CELLS EXPOSED TO AUJESZKY’S DISEASE VIRUS (PSEUDORABIES VIRUS). African Journal of Traditional, Complementary and Alternative Medicines, 12(4), 106–111. https://doi.org/10.21010/ajtcam.v12i4.16

Issue

Section

Research Papers