DIPEPTIDYL PEPTIDASE IV INHIBITORY ACTIVITY OF PROTEIN HYDROLYZATES FROM AMARANTHUS HYPOCHONDRIACUS L. GRAIN AND THEIR INFLUENCE ON POSTPRANDIAL GLYCEMIA IN STREPTOZOTOCIN-INDUCED DIABETIC MICE.

Authors

  • Jorge Soriano-Santos Universidad Autónoma Metropolitana-Iztapalapa
  • Raúl Reyes-Bautista Universidad Autónoma Metropolitana-Iztapalapa
  • Isabel Guerrero-Legarreta Universidad Autónoma Metropolitana-Iztapalapa
  • Edith Ponce-Alquicira Universidad Autónoma Metropolitana-Iztapalapa
  • Héctor Bernardo Escalona-Buendía Universidad Autónoma Metropolitana-Iztapapala
  • Julio César Almanza-Pérez Universidad Autónoma Metropolitana-Iztapalapa
  • Gerardo Díaz-Godínez Universidad Autónoma de Tlaxcala
  • Rubén Román-Ramos Universidad Autónoma Metropolitana Iztpalapa

DOI:

https://doi.org/10.21010/ajtcam.v12i1.13

Keywords:

Amaranth protein hydrolyzate, DPP-IV inhibitory activity, diabetes

Abstract

Background: Type 2 diabetes is a chronic metabolic disorder. Recently, dipeptidyl peptidase IV (DPP-IV) inhibitors that protect incretin hormones from being cleaved by DPP-IV have been used as drugs to control glycemia. This study examined the potential hypoglycemic effect of amaranth grain storage protein hydrolyzates to control postprandial glycemia in streptozotocin (STZ)-induced diabetic mice as a model system of diabetes, and their inhibition mode on the enzyme. Material and Methods: Amaranth grain proteins were isolated and hydrolyzed and fractionated by gel filtration. The DPP-IV inhibitory activity of hydrolyzates as well as their kinetic parameters were assessed. Selected hydrolyzates (300 mg/kg body weight) were administered in a single administration-study (SAS) or in the same concentration during a four-week chronic daily dosing study (FWCDDS) in order to observe the effect on postprandial glycemia of diabetic mice. Results: Albumin 1, Globulin and Glutelin hydrolyzates (GluH) competitively inhibited DPP-IV in vitro (Ki= 0.11-5.61 mg/mL). GluH called Glu.III (IC50= 0.12±0.01 mg/mL) considerably inhibited DPP-IV activity. GluH identified as GluH24 improved glucose tolerance significantly (p<0.05), with remarkable increments in plasma insulin in SAS and FWCDDS (1.25 and 2.25 mg/mL, respectively). This effect could be compared to the one obtained from the mice group that was administered Sitagliptin (580 mg/kg body weight) as positive control (p<0.05). Conclusion: Amaranth Glutelin hydrolyzates yielded the highest enzyme inhibitory activity reported not only in vitro, but also in the STZ-induced diabetic mice in order to control postprandial glycemia.

Author Biographies

Jorge Soriano-Santos, Universidad Autónoma Metropolitana-Iztapalapa

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Raúl Reyes-Bautista, Universidad Autónoma Metropolitana-Iztapalapa

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Isabel Guerrero-Legarreta, Universidad Autónoma Metropolitana-Iztapalapa

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Edith Ponce-Alquicira, Universidad Autónoma Metropolitana-Iztapalapa

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Héctor Bernardo Escalona-Buendía, Universidad Autónoma Metropolitana-Iztapapala

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Julio César Almanza-Pérez, Universidad Autónoma Metropolitana-Iztapalapa

Dr. PhD. Departamento de Biotechnología. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

Gerardo Díaz-Godínez, Universidad Autónoma de Tlaxcala

Dr. PhD. Laboratorio de Biotecnología, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, CP 90000, Mexico

Rubén Román-Ramos, Universidad Autónoma Metropolitana Iztpalapa

Dr. PhD. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, No. 186, Col. Vicentina, Ap. P. 55-535, Deleg. Iztapalapa, CP 09340, Mexico City, Mexico

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Published

2014-11-29

How to Cite

Soriano-Santos, J., Reyes-Bautista, R., Guerrero-Legarreta, I., Ponce-Alquicira, E., Escalona-Buendía, H. B., Almanza-Pérez, J. C., Díaz-Godínez, G., & Román-Ramos, R. (2014). DIPEPTIDYL PEPTIDASE IV INHIBITORY ACTIVITY OF PROTEIN HYDROLYZATES FROM AMARANTHUS HYPOCHONDRIACUS L. GRAIN AND THEIR INFLUENCE ON POSTPRANDIAL GLYCEMIA IN STREPTOZOTOCIN-INDUCED DIABETIC MICE. African Journal of Traditional, Complementary and Alternative Medicines, 12(1), 90–98. https://doi.org/10.21010/ajtcam.v12i1.13

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Section

Case Report