IN VITRO IMMUNE-MODULATORY POTENTIAL OF CRUDE EXTRACT OF LEAFS OF ALBIZIA GUMMIFERA AGAINST STIMULATED PERIPHERAL BLOOD MONONUCLEAR AND RAW CELLS

Authors

  • Marema Ephraim Makgatho
  • Aubrey Mashele
  • Game Makhubele
  • Moditi Sedibane
  • Felistas Mashinya
  • Matlou Mokgotho
  • Thabe Matsebatlela
  • Robert Makola

DOI:

https://doi.org/10.21010/ajtcam.v12i3.10

Keywords:

Albizia gummifera, Interleukin-2, nitric oxide

Abstract

The effect of crude leaf extract of Albizia gummifera (5-160µg/ml) was evaluated on cytotoxic activity against peripheral blood mononuclear and RAW cells stimulated with phorbol myristate acetate (25ng/ml) and formyl peptides (1µM) respectively using the WST-1 technique. Nitric oxide production by RAW cells and Intereukin-2 (IL-2) production by PBMC were also assessed at the same crude leaf extract concentrations (5-160µg/ml) employing colorimetric procedures. The plant leaf extract dose-dependently inhibited growth of mononuclear cells while promoting that of RAW cells (p<0.05). Interleikin-2 production by mononuclear cells and nitric oxide release by RAW cells were inhibited and promoted respectively (p<0.05). Phytochemical studies showed that the leaf extract tested positive for flavonoids, saponins and tannins. This is a preliminary study that showed significant anti- and pro-inflammatory potential at concentration above 20 and 40µg/ml respectively. Further laboratory research is essential to elucidate the anti- and pro-inflammatory biochemical pathways as shown by the in vitro immune-modulatory modalities of this plant species. It is also important to further identify bioactive entities of Albizia gummifera responsible for the observed activities.

Author Biography

Marema Ephraim Makgatho

Medical Sciences, Senior Lecturer

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Published

2015-04-04

How to Cite

Makgatho, M. E., Mashele, A., Makhubele, G., Sedibane, M., Mashinya, F., Mokgotho, M., Matsebatlela, T., & Makola, R. (2015). IN VITRO IMMUNE-MODULATORY POTENTIAL OF CRUDE EXTRACT OF LEAFS OF ALBIZIA GUMMIFERA AGAINST STIMULATED PERIPHERAL BLOOD MONONUCLEAR AND RAW CELLS. African Journal of Traditional, Complementary and Alternative Medicines, 12(3), 85–90. https://doi.org/10.21010/ajtcam.v12i3.10

Issue

Section

Research Papers