ANTI-BACTERIAL AND ANTI-OXIDANT ACTIVITIES OF LEAF EXTRACTS OF COMBRETUM VENDAE (COMBRETECACEA) AND THE ISOLATION OF AN ANTI-BACTERIAL COMPOUND.

Authors

  • Nancy Patience Motlalepula Komape Univeristy of Limpopo
  • Mutalib Aderogba
  • Victor Patrick Bagla
  • Peter Masoko
  • Jacobus Nicolaas Eloff

Keywords:

Combretum vendae, Minimum Inhibitory Concentration, Bio-autography, acacetin, Phytochemical analysis, Anti-oxidant

Abstract

Background: Combretum vendae A.E. van Wyk (Combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of South Africa. Dried leaves extracts of C. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated. Materials and methods: Constituents of leaf material were serially extracted using solvents of varying polarities, TLC chromatograms of the fractions were sprayed with 2,2 diphenyl-1-picrylhydrazyl (DPPH) to determine the presence of antioxidant compounds. Bio-autography was used to determine the number of antibacterial compounds active against Staphylococcus aureus, Enterococcus faecalis, Eschericha coli and Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) values were determined using serial microplate dilution method. The chloroform fraction was subjected to bio-assay guided column chromatography to isolate the active compound. Results: The mass extracted by different solvents was below 10% dry weight. MIC values for different extracts against different pathogens ranges from 0.08 to 0.64 mg/ml. The compound isolated was identified as acacetin having an Rf value of 0.28 following elution in the Ethanol: Methanol: Water [E: M: W (10: 1.35: 1 v/v). Acacetin had MIC values ranging from 0.16 to 0.35 mg/ml. Conclusion: We report for the first time the isolation of acacetin as the main antibacterial compound from the leaves of Combretum vendae.

Author Biographies

Nancy Patience Motlalepula Komape, Univeristy of Limpopo

Microbiology and Professor

Peter Masoko

2University of Limpopo, Department of Biochemistry, Microbiology and Biotechnology, Private Bag X1106, Sovenga, 0727

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Published

2014-08-18

How to Cite

Komape, N. P. M., Aderogba, M., Bagla, V. P., Masoko, P., & Eloff, J. N. (2014). ANTI-BACTERIAL AND ANTI-OXIDANT ACTIVITIES OF LEAF EXTRACTS OF COMBRETUM VENDAE (COMBRETECACEA) AND THE ISOLATION OF AN ANTI-BACTERIAL COMPOUND. African Journal of Traditional, Complementary and Alternative Medicines, 11(5), 73–77. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/2227

Issue

Section

Research Papers