PHYTOCHEMICAL AND IN VITRO ANTIMICROBIAL ASSAY OF THE LEAF EXTRACT OF NEWBOULDIA LAEVIS

Authors

  • Hamidu USMAN UNIVERSITY of MAIDUGURI
  • Jabir Chukwuemeka Osuji UNIVERSITY of MAIDUGURI

Keywords:

Antimicrobial, Extracts, In-Vitro, Leaf, Phytochemical, Newbouldia leavis

Abstract

The methanolic leaf extract of Newbouldia laevis was subjected to preliminary phytochemical screening and in-vitro antimicrobial tests. The extract revealed the presence of flavonoids, tannins, terpenes, steroidal and cardiac glycosides. The antimicrobial activity of the plant extract was assayed by the agar plate disc diffusion and nutrient broth dilution techniques. Test microorganisms were Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella spp. and Candida albicans; all the organisms were laboratory isolates. The extract inhibited the growth of all the test organisms especially against Klebsiella spp. and S. aureus which had mean inhibition zone of 42.3±1.5 and 32.3±1.5 mm respectively. The results showed minimum inhibitory concentration (MIC) of 1.563 mg/ml against Escherichia coli and Klebsiella spp. and 3.125 mg/ml against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. The minimal bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus was 0.39 mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhea, dysentery and as a remedy for wound healing whose causative agents are some of the organisms used in this study.

Author Biographies

Hamidu USMAN, UNIVERSITY of MAIDUGURI

Department of Chemistry (ASSISTANT LECTURER)

Jabir Chukwuemeka Osuji, UNIVERSITY of MAIDUGURI

Department of Chemistry

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Published

2007-06-10

How to Cite

USMAN, H., & Osuji, J. C. (2007). PHYTOCHEMICAL AND IN VITRO ANTIMICROBIAL ASSAY OF THE LEAF EXTRACT OF NEWBOULDIA LAEVIS. African Journal of Traditional, Complementary and Alternative Medicines, 4(4), 476 –. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/209

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Section

Research Papers