IN VITRO ASSESSMENT OF ANTIOXIDANT, PHYTOCHEMICAL AND NUTRITIONAL PROPERTIES OF EXTRACTS FROM THE LEAVES OF OCIMUM GRATISSIMUM (LINN)

Authors

  • Etinosa O. Igbinosa
  • Edwina O. Uzunuigbe
  • Isoken H. Igbinosa
  • Emmanuel E. Odjadjare
  • Nicholas O. Igiehon
  • Oke A. Emuedo

Keywords:

Ocimum gratissimum, oxidative stress, polyphenolic, proximate composition, therapeutic activity

Abstract

The antioxidant, phytochemical and nutritional properties of acetone, methanol and aqueous extracts of the leaves of Ocimum gratissimum (Linn) were investigated to evaluate the therapeutic and nutritional potential of the leaves of this plant. The antioxidant of the plant extracts were assessed against 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing agent. Total phenolics, flavonoids, flavonols and proanthocyanidins were determined to assess their corresponding effect on the antioxidant activity of this plant. The extracts exhibited DPPH and ABTS.+ radical scavenging activities, which was comparable to butylated hydroxytoluene (BHT). The phytochemical screening revealed the presence of alkaloids, tannins, saponin, steroids, cardiacglycoside, flavonoid, terpenoids and phenol. The proximate analysis confirms that the leaves contain appreciable amount of ash, crude protein, lipids, fibre and carbohydrates. The macro and micro elements and constituents revealed that the leaves contain significant amount of sodium, potassium, calcium, magnesium, iron, zinc, phosphorus, copper, nitrogen, and manganese. This study shows that the leaf can be used as a therapeutic agent and justifies its application in folkloric medicine.

Author Biography

Etinosa O. Igbinosa

Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, Nigeria

Downloads

Published

2013-07-02

How to Cite

Igbinosa, E. O., Uzunuigbe, E. O., Igbinosa, I. H., Odjadjare, E. E., Igiehon, N. O., & Emuedo, O. A. (2013). IN VITRO ASSESSMENT OF ANTIOXIDANT, PHYTOCHEMICAL AND NUTRITIONAL PROPERTIES OF EXTRACTS FROM THE LEAVES OF OCIMUM GRATISSIMUM (LINN). African Journal of Traditional, Complementary and Alternative Medicines, 10(5), 292–298. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1943

Issue

Vol. 10 No. 5 (2013)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.