DETERMINATION OF PESTICIDE RESIDUES IN KHAT LEAVES BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • Ali N. A. Awadh Pharmacognosy Department, Faculty of Medicine and Health Sciences, Sana'a, Ajman, U. A. E.
  • D. Lemme
  • Th. Jira
  • O. Attef
  • K. Al-rahwi

Keywords:

Khat leaves, methidathion, penconazole, dimethoate, pesticides, Catha edulis

Abstract

The purpose of this investigation is to develop a simple method for the assessment of pesticides in Khat leaves, Catha edulis FORSK. (Celastraceae) shrub, chewed by about 60 % of the Yemeni people, producing amphetamine-like psychostimulation. The first procedures involve solid-phase extraction using Adsorbex® RP-C18 and Bakerbond® RP-C18 disposable cartridges with 40 % acetonitrile for elution. Isocratic and gradient analysis was carried out by means of high-performance liquid chromatography using different stationary phases to analyse methidathion, peconazole and dimethoate. An UltrasphereODS RP-18-column with UV-detection at 210 nm and 80/20 % (v/v) acetonitrile/water as mobile phase (isocratic) was used to determine the content of methidathion, peconazole and dimethoate (detection limit 20-25 ng). The method was validated with fortified samples at pesticide concentration of 0.1 mg/ml. Average recoveries ranging from 97.9 and 101.6% with a relative standard deviation (RSD) of 1.7-3.8 %. Each recovery analysis was repeated four times. Limits of detection ranged from the analytical procedures were applied to 5 samples of khat and no detectable amounts of pesticides were found in any sample under the conditions described.

Author Biographies

Ali N. A. Awadh, Pharmacognosy Department, Faculty of Medicine and Health Sciences, Sana'a, Ajman, U. A. E.

Pharmacognosy Department, Faculty of Medicine and Health Sciences, Sana'a University

D. Lemme

Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Ernst-Moritz-Arndt-University, D-17487 Greifswald, Germany

Th. Jira

Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Ernst-Moritz-Arndt-University, D-17487 Greifswald, Germany

O. Attef

Department of Pharmacognosy and Pharmaceutical Chemistry, Ajman University of Science and Technology Network, Ajman, U. A. E

K. Al-rahwi

Pharmacognosy Department, Faculty of Medicine and Health Sciences, Sana'a University

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Published

2006-01-12

How to Cite

Awadh, A. N. A., Lemme, D., Jira, T., Attef, O., & Al-rahwi, K. (2006). DETERMINATION OF PESTICIDE RESIDUES IN KHAT LEAVES BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. African Journal of Traditional, Complementary and Alternative Medicines, 3(1), 1–10. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/19

Issue

Vol. 3 No. 1 (2006)

Section

Research Papers

License

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