THE ANTI-TUMOR EFFECT AND BIOLOGICAL ACTIVITIES OF THE EXTRACT JMM6 FROM THE STEM-BARKS OF THE CHINESE JUGLANS MANDSHURICA MAXIM ON HUMAN HEPATOMA CELL LINE BEL-7402

Authors

  • Yongli Zhang
  • Yuqiang Cui
  • Jiayong Zhu
  • Hongzhi Li
  • Jianwen Mao
  • Xiaobao Jin
  • Xiangsheng Wang
  • Yifan Du
  • Jiazheng Lu

Keywords:

Juglans mandshurica Maxim, stem-barks, anti-tumor, apoptosis, mitochondrial membrane potential, cell cycle.

Abstract

Juglans mandshurica Maxim is a traditional herbal medicines in China, and its anti-tumor bioactivities are of research interest. Bioassay-guided fractionation method was employed to isolate anti-tumor compounds from the stem barks of the Juglans mandshurica Maxim. The anti-tumor effect and biological activities of the extracted compound JMM6 were studied in BEL-7402 cells by MTT, Cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay and Detection of mitochondrial membrane potential (△Ψm). After treatment with the JMM6, the growth of BEL-7402 cells was inhibited and cells displayed typical morphological apoptotic characteristics. Further investigations revealed that treatment with JMM6 mainly caused G2/M cell cycle arrest and induced apoptosis in BEL-7402 cells. To evaluate the alteration of mitochondria in JMM6 induced apoptosis. The data showed that JMM6 decreased significantly the △Ψm, causing the depolarization of the mitochondrial membrane. Our results show that the JMM6 will have a potential advantage of anti-tumor, less harmful to normal cells. This paper not only summarized the JMM6 pick-up technology from Juglans mandshurica Maxim and biological characteristic, but also may provide further evidence to exploit the potential medicine compounds from the stem-barks of the Chinese Juglans mandshurica Maxim.

Author Biography

Yongli Zhang

Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China

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Published

2012-12-11

How to Cite

Zhang, Y., Cui, Y., Zhu, J., Li, H., Mao, J., Jin, X., Wang, X., Du, Y., & Lu, J. (2012). THE ANTI-TUMOR EFFECT AND BIOLOGICAL ACTIVITIES OF THE EXTRACT JMM6 FROM THE STEM-BARKS OF THE CHINESE JUGLANS MANDSHURICA MAXIM ON HUMAN HEPATOMA CELL LINE BEL-7402. African Journal of Traditional, Complementary and Alternative Medicines, 10(2), 258–269. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1726

Issue

Vol. 10 No. 2 (2013)

Section

Research Papers

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