ANTI-INFLAMMATORY, CYTOTOXIC AND ANTIOXIDANT EFFECTS OF METHANOLIC EXTRACTS OF DRYPETES SEPIARIA (EUPHORBIACEAE)

Authors

  • Ganesh Gadamsetty VIT University
  • Saurabh Maru Shri G S Institute of Technology and Science, Indore
  • Abhishek Tyagi VIT University
  • Sarada Nallani Chakravarthula School of Advanced Sciences, VIT University, Vellore-14, Tamil Nadu, India

Keywords:

Drypetes sepiaria, Euphorbiaceae, Anti-oxidant, Anti-inflammatory, Cytotoxicity, Apoptosis

Abstract

The aim of this study was to investigate in vitro anti-oxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of Drypetes sepiaria (Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In in vitro antioxidant method methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC50 of 95.43µg/ml (DPPH) and 67.05µg/ml (ABTS). Methanol extract was able to inhibit inflammation by in vitro about 85-90% (HRBC stabilization method) and in vivo about 40-45% (Paw edema method) anti-inflammatory assays compared to standard produced 50.04% at 6h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC50 of 10µg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24% and increased up to 40% after treatment.

Author Biographies

Ganesh Gadamsetty, VIT University

School of Advanced Sciences

Saurabh Maru, Shri G S Institute of Technology and Science, Indore

Department of Pharmacy

Abhishek Tyagi, VIT University

School of Advanced Sciences

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Published

2013-07-02

How to Cite

Gadamsetty, G., Maru, S., Tyagi, A., & Chakravarthula, S. N. (2013). ANTI-INFLAMMATORY, CYTOTOXIC AND ANTIOXIDANT EFFECTS OF METHANOLIC EXTRACTS OF DRYPETES SEPIARIA (EUPHORBIACEAE). African Journal of Traditional, Complementary and Alternative Medicines, 10(5), 274–282. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1674

Issue

Vol. 10 No. 5 (2013)

Section

Research Papers

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