ADVANCES IN THE ANALYTICAL METHODS FOR DETERMINING THE ANTIOXIDANT PROPERTIES OF HONEY: A REVIEW

Authors

  • M Moniruzzaman
  • M I Khalil
  • S A Sulaiman
  • S H Gan

Keywords:

Honey, antioxidant properties, DPPH, FRAP, ORAC, TEAC, ABTS

Abstract

Free radicals and reactive oxygen species (ROS) have been implicated in contributing to the processes of aging and disease. In an effort to combat free radical activity, scientists are studying the effects of increasing individuals' antioxidant levels through diet and dietary supplements. Honey appears to act as an antioxidant in more ways than one. In the body, honey can mop up free radicals and contribute to better health. Various antioxidant activity methods have been used to measure and compare the antioxidant activity of honey. In recent years, DPPH (Diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), ORAC (The Oxygen Radical Absorbance Capacity), ABTS [2, 2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid) diamonium salt], TEAC [6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (Trolox)-equivalent antioxidant capacity] assays have been used to evaluate antioxidant activity of honey. The antioxidant activity of honey is also measured by ascorbic acid content and different enzyme assays like Catalase (CAT), Glutathione Peroxidase (GPO), Superoxide Dismutase (SOD). Among the different methods available, methods that have been validated, standardized and widely reported are recommended.

Author Biographies

M Moniruzzaman

Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

S H Gan

Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

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Published

2011-08-04

How to Cite

Moniruzzaman, M., Khalil, M. I., Sulaiman, S. A., & Gan, S. H. (2011). ADVANCES IN THE ANALYTICAL METHODS FOR DETERMINING THE ANTIOXIDANT PROPERTIES OF HONEY: A REVIEW. African Journal of Traditional, Complementary and Alternative Medicines, 9(1), 36–42. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1354

Issue

Vol. 9 No. 1 (2012)

Section

Review

License

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