ANALYSIS OF THE EXTRACTS OF ISATIS TINCTORIA BY NEW ANALYTICAL APPROACHES OF HPLC, MS AND NMR

Authors

  • Jue Zhou
  • Fan Qu

Keywords:

Isatis tinctoria, HPLC, MS, NMR, indole glucosinolates (GLs), alkaloids.

Abstract

The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) of Isatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.

Author Biographies

Jue Zhou

Department of Pharmacy, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom

Fan Qu

The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

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Published

2011-04-17

How to Cite

Zhou, J., & Qu, F. (2011). ANALYSIS OF THE EXTRACTS OF ISATIS TINCTORIA BY NEW ANALYTICAL APPROACHES OF HPLC, MS AND NMR. African Journal of Traditional, Complementary and Alternative Medicines, 8(5S), 33–45. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1307

Issue

Vol. 8 No. 5S (2011)

Section

Research Papers

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