EXPERIMENTAL DESIGN OPTIMIZATION FOR SCREENING RELEVANT FREE PHENOLIC ACIDS FROM VARIOUS PREPARATIONS USED IN BURKINA FASO FOLK MEDICINE

Authors

  • Martin Kiendrebeogo
  • Luc Choisnard
  • Charles E. Lamien
  • Aline Meda
  • Denis Wouessidjewe
  • Odile G Nacoulma

Keywords:

Central Composite Face-Centred Design, Chromatographic Response Function, Phenolic acids

Abstract

Experimental design methodology was used to optimize the HPLC separation of various relevant phenolic acids from an artificial mixture. The effect of four characteristic factors of the HPLC procedure on the Chromatographic Response Function was investigated by a Central Composite Face-Centred Design and Multi Linear Regression fitting. The eight phenolic acids were successfully separated. The optimal HPLC setting condition was applied to rapid screening of free phenolic acids from Striga hermonthica (Del.) Benth. (Scrophulariaceae), Guiera senegalensis J. F. Gmel (Combretaceae) and unifloral honey from Acacia sp. (Mimosaceae). None of reference phenolic acid was identified in S. hermonthica extract when some of them were detected in G. senegalensis and honey extracts. This work is considered as a first step for a largest phenolic acids screening from various preparations used in Burkina Faso folk medicine to justify some of their properties or potentialities.

Author Biographies

Martin Kiendrebeogo

Laboratoire de Biochimie et de Chimie Appliquées, UFR Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso

Luc Choisnard

Département de Pharmacochimie Moléculaire (DPM), UMR CNRS 5063, Pharmacotechnie, Université Joseph Fourier, 38243 Meylan Cedex, France

Charles E. Lamien

Laboratoire de Biochimie et de Chimie Appliquées, UFR Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso

Aline Meda

Laboratoire de Biochimie et de Chimie Appliquées, UFR Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso

Denis Wouessidjewe

Département de Pharmacochimie Moléculaire (DPM), UMR CNRS 5063, Pharmacotechnie, Université Joseph Fourier, 38243 Meylan Cedex, France

Odile G Nacoulma

Laboratoire de Biochimie et de Chimie Appliquées, UFR Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso

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Published

2006-01-12

How to Cite

Kiendrebeogo, M., Choisnard, L., Lamien, C. E., Meda, A., Wouessidjewe, D., & Nacoulma, O. G. (2006). EXPERIMENTAL DESIGN OPTIMIZATION FOR SCREENING RELEVANT FREE PHENOLIC ACIDS FROM VARIOUS PREPARATIONS USED IN BURKINA FASO FOLK MEDICINE. African Journal of Traditional, Complementary and Alternative Medicines, 3(1), 115–128. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/13

Issue

Vol. 3 No. 1 (2006)

Section

Research Papers

License

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