EFFECT OF ACHYRANTHES BIDENTATA POLYSACCHARIDES ON THE EXPRESSION OF BCL-2 AND BAX IN HEPATIC TISSUES AFTER EXHAUSTIVE EXERCISE IN RATS

Authors

  • J. Lin
  • Z. Zhang
  • Y. Shan

Keywords:

Achyranthes bidentata polysaccharides, apoptosis, bcl-2, bax, exhaustive exercise

Abstract

This study aims to assess the effects of Achyranthes bidentata polysaccharides (ABPS) on the expression of bcl-2 and bax in hepatic tissues after exhaustive exercise in order to provide theoretical support for the application of ABPS in the field of sports nutrition. Thirty male Sprague-Dawley rats were randomized into three groups, each consisting of 10 rats: Normal control group (NCG), Exhausting exercises control group (EECG), ABPS treated group (ATG). ABPS were fed orally by gastric intubation to rats of ABPS treated group (ATG) once daily for 7 days. Control animals (EECG and NCG) received the same amount of isotonic sodium chloride solution. Exhaustive exercise was performed on a rodent treadmill. The SP (streptavidin peroxidase) method for immunohistochemical staining was adopted to test the protein expression of bax and bcl-2 in the hepatic tissues of the rats. Exhausting exercises increased bax protein expression of hepatic tissues of rats and bax/bcl-2 ratio dramatically, but a decreased bcl-2 protein expression. In the rats fed ABPS orally by gastric intubation, the bax protein expression and bax/bcl-2 ratio obviously decreased, while bcl-2 protein expression increased. The result indicated that bax and bcl-2 co-regulated the exercise-induced hepatocyte apoptosis. Feeding ABPS orally by gastric intubation to rats can inhibit the hepatocyte apoptosis in exhaustive exercise.

Author Biography

J. Lin

Department of Physical Education, Central South University, Changsha City, Hunan Province, 410083, China

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Published

2010-05-07

How to Cite

Lin, J., Zhang, Z., & Shan, Y. (2010). EFFECT OF ACHYRANTHES BIDENTATA POLYSACCHARIDES ON THE EXPRESSION OF BCL-2 AND BAX IN HEPATIC TISSUES AFTER EXHAUSTIVE EXERCISE IN RATS. African Journal of Traditional, Complementary and Alternative Medicines, 7(4), 307–314. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1112

Issue

Vol. 7 No. 4 (2010)

Section

Research Papers

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