IN-VITRO EVALUATION OF ANTI-TRICHOMONAL ACTIVITIES OF EUGENIA UNIFLORA LEAF.

Authors

  • Adeleke Clement ADEBAJO Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.
  • Gabriel Femi Ibikunle Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.
  • Funmilayo Gladys Famuyiwa Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.
  • Adetunji Joseph Aladesanmi Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.
  • Clement Oladapo Adewunmi Drug Research and Development Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Nigeria.

Keywords:

Eugenia uniflora, leaf extract, Trichomonas gallinae, in vitro.

Abstract

Eugenia uniflora, used ethnomedically in some tropical countries as an anti-infective, has shown anti-malarial and anti-trypanocidal activities. Therefore using bioactivity guided fractionation, anti-trichomonal activity of E. uniflora leaf was investigated. Anti-trichomonal activities of leaf methanol extract and its fractions against Trichomonas gallinae as well as their cytotoxicities using an in vitro haemaglutination assay were determined. Anti-trichomonacidal activities of the extract improved on purification up to a stage. Subfractions E2-5 had LC50 and LC90 values of 4.77 - 5.28, 18.49 - 25.00 and 4.53 - 5.18, 18.32 - 19.07 μg/ml at 24 and 48 hrs, respectively that were better than those of metronidazole. Further purification of E2-5 led to loss of activity suggesting that the active components were probably working synergistically and additively. Demonstration of low haemaglutination titre values of 0.00 – 5.33 by methanolic extract and its partition fractions suggested their low toxicity profile. The established safety of the leaf indicated that its anti-trichomonal activity was not due to non-specific cytotoxicity, hence could be used in ethnomedicine as an anti-trichomonal agent.

Author Biographies

Adeleke Clement ADEBAJO, Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria. Senior Lecturer

Gabriel Femi Ibikunle, Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria. Postgraduate Student

Funmilayo Gladys Famuyiwa, Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria. Postgraduate Student

Adetunji Joseph Aladesanmi, Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria.

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Osun State. Nigeria. Professor

Clement Oladapo Adewunmi, Drug Research and Development Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Nigeria.

Drug Research and Development Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife. Nigeria. Professor

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Published

2010-11-09

How to Cite

ADEBAJO, A. C., Ibikunle, G. F., Famuyiwa, F. G., Aladesanmi, A. J., & Adewunmi, C. O. (2010). IN-VITRO EVALUATION OF ANTI-TRICHOMONAL ACTIVITIES OF EUGENIA UNIFLORA LEAF. African Journal of Traditional, Complementary and Alternative Medicines, 8(2), 170–176. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/1055

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Section

Research Papers

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