SYNTHESIS AND INHIBITORY EFFECT OF SYZYGIUM AROMATICUM ON STREPTOCOCCUS PYOGENES-A MULTI DRUG RESISTANCE BACTERIUM
DOI:
https://doi.org/10.21010/Ajidv20i1.2%20Keywords:
Nanoparticles, chronic diseases, bacterial inhibition, cloves, Streptococcus pyogenes, callusAbstract
Background: Multidrug-resistance bacteria distinctly threaten world health through the promotion of infectious diseases and an increase in the mortality rate. Nanotechnology represents a novel solution that employs nanoscale materials with distinct physicochemical and biological properties. Among these materials, silver nanoparticles are widely noted for their potent antimicrobial activity and eco-friendly synthesis via natural plant extracts, such as Syzygium aromaticum (clove). The present study evaluated the antibacterial efficacy of biosynthesized silver nanoparticles from S. aromaticum extract against MDR Streptococcus pyogenes isolated from chronic tonsillitis patients.
Materials and Methods: Swab samples from chronic tonsillitis patients were cultured on blood and nutrient agar to isolate S. pyogenes, which was further confirmed by biochemical assays. Biosynthesis of silver nanoparticles was performed using a green process with S. aromaticum extract, and antibacterial effectiveness was analyzed by the Kirby-Bauer disk diffusion method at different concentrations.
Results: Characterization studies confirmed that the biosynthesized silver nanoparticles (AgNPs) met the physicochemical criteria of nanomaterials, including sizes less than 100 nm, defined shape, high surface area, stabilized surface charge, and characteristic optical properties, including absorption in the 400 to 450 nm wavelength range. The AgNPs synthesized by the bio process showed good antibacterial activity, with inhibition zone measurements of 11.8 ±1.3, 14.2 ±2.1, 16.4 ±1.5, and19.8 ±1.5 at 25,50,75,and 100 percent concentrations, indicating a significant dose effect (p<0.05).
Conclusion: Green-synthesized AgNPs from Syzygium aromaticum showed marked inhibitory action against MDR S. pyogenes and can therefore be recommended as cost-effective, sustainable, and eco-friendly alternatives in the management of resistant bacterial infections.
References
Amooaghaie, R., Saeri, M. R., and Azizi, M. (2015). Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles. Ecotoxicology and Environmental Safety, 120, 400–408.
Awad, M. A., Eid, A. M., Elsheikh, T. M. Y., Al-Faifi, Z. E., Saad, N., Sultan, M. H., (2022). Mycosynthesis, characterization, and mosquitocidal activity of silver nanoparticles fabricated by Aspergillus niger strain. Journal of Fungi, 8, 396.
Bulla, S. S., Bhajantri, R. F., and Chavan, C. (2021). Optical and structural properties of biosynthesized silver nanoparticle encapsulated PVA (Ag–PVA) films. Journal of Inorganic and Organometallic Polymers and Materials, 31(6), 2368-2380.
Clinical and Laboratory Standards Institute (CLSI). (2015). Performance standards for antimicrobial disk susceptibility tests for bacteria isolated from animals: CLSI supplement VET01S. Wayne, PA: CLSI.
Darabdhara, J., and Ahmaruzzaman, M. (2022). Metal oxide-biochar nanocomposites for the effective removal of environmental contaminants. In Advanced Materials for a Sustainable Environment (pp. 143–158). Boca Raton, FL: CRC Press.
Demchenko, V., Kobylinskyi, S., Iurzhenko, M., Riabov, S., Vashchuk, A., Rybalchenko, N., (2022). Nanocomposites based on polylactide and silver nanoparticles and their antimicrobial and antiviral applications. Reactive and Functional Polymers, 170, 105096.
Dhandapani, K. V., Anbumani, D., Gandhi, A. D., Annamalai, P., Muthu Venkatachalam, B. S., Kavitha, P.,(2020). Green route for the synthesis of zinc oxide nanoparticles from Melia azedarach leaf extract and evaluation of their antioxidant and antibacterial activities. Biocatalysis and Agricultural Biotechnology, 24, 101517.
Eker, F., Akdaşçi, E., Duman, H., Bechelany, M., and Karav, S. (2025). Green synthesis of silver nanoparticles using plant extracts: A comprehensive review of physicochemical properties and multifunctional applications. International Journal of Molecular Sciences, 26(13), 6222. https://doi.org/10.3390/ijms26136222
El Faqer, O., Bendiar, S., Rais, S., Elkoraichi, I., Dakir, M., Elouaddari, A., (2022). Phytochemical characterization and immunomodulatory effects of aqueous, ethanolic extracts and essential oil of Syzygium aromaticum L. on human neutrophils. Scientific African, 18, e01395.
Erkoc, P. (2021). Sodium borohydride and essential oils as reducing agents for the chemically and green synthesis of silver nanoparticles: a comparative analysis. Journal of the Turkish Chemical Society Section A: Chemistry, 8(1), 1-8.
Esposito, S., Masetti, M., Calanca, C., Canducci, N., Rasmi, S., Fradusco, A., and Principi, N. (2025). Recent changes in the epidemiology of group A streptococcus infections: Observations and implications. Microorganisms, 13 (8), 1871. https://doi.org/10.3390/microorganisms13081871
Ghosh, S., Patil, S., Ahire, M., Kitture, R., Kale, S., Pardesi, K., (2012). Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. International Journal of Nanomedicine, 7, 483–496.
Gomaa, E. Z. (2017). Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria. Journal of General and Applied Microbiology, 63, 36–43.
González-Pedroza, M. G., Argueta-Figueroa, L., García-Contreras, R., Jiménez-Martínez, Y., Martínez-Martínez, E., Navarro-Marchal, S. A., (2021). Silver nanoparticles from Annona muricata peel and leaf extracts as a potential potent, biocompatible and low-cost antitumor tool. Nanomaterials, 11, 1273.
Gupta, D., Boora, A., Thakur, A., and Gupta, T. K. (2023). Green and sustainable synthesis of nanomaterials: Recent advancements and limitations. Environmental Research, 231, 116316. https://doi.org/10.1016/j.envres.2023.116316
Huq, M. A., Ashrafudoulla, M., Rahman, M. M., Balusamy, S. R., and Akter, S. (2022). Green synthesis and potential antibacterial applications of bioactive silver nanoparticles: A review. Polymers, 14, 742.
Kaga, S., Truong, N. P., Esser, L., Senyschyn, D., Sanyal, A., Sanyal, R., (2017). Influence of size and shape on the biodistribution of nanoparticles prepared by polymerization-induced self-assembly. Biomacromolecules, 18(12), 3963–3970.
Kim, Y. H., Lee, D. K., Cha, H. G., Kim, C. W., Kang, Y. C., and Kang, Y. S. (2006). Preparation and characterization of antibacterial Cu nanoparticles formed on the surface of SiO₂ nanoparticles. Journal of Physical Chemistry B, 110(49), 24923–24928.
Kumar, S. S., Venkateswarlu, P., Rao, V. R., and Rao, G. N. (2013). Synthesis, characterization and optical properties of zinc oxide nanoparticles. International Nano Letters, 3, 1–6.
Liu, J., Ma, Z., Liu, Y., Zheng, X., Pei, Y., and Tang, K. (2022). Soluble soybean polysaccharide films containing in-situ generated silver nanoparticles for antibacterial food packaging applications. Food Packaging and Shelf Life, 31, 100800.
Manivasagan, P., Venkatesan, J., Senthilkumar, K., Sivakumar, K., and Kim, S. K. (2013). Biosynthesis, antimicrobial and cytotoxic effect of silver nanoparticles using a novel Nocardiopsis sp. MBRC-1. BioMed Research International, 2013, 287638.
Morales-Luckie, R. A., Sanchez-Mendieta, V., Arenas-Alatorre, J. A., López-Castañares, R., Perez-Mazariego, J. L., Marquina-Fabrega, V., et al. (2008). One-step aqueous synthesis of stoichiometric Fe–Cu nanoalloy. Materials Letters, 62, 4195–4197.
Mystrioti, C., Xanthopoulou, T., Tsakiridis, P., Papassiopi, N., and Xenidis, A. (2016). Comparative evaluation of five plant extracts and juices for nanoiron synthesis and application for hexavalent chromium reduction. Science of the Total Environment, 539, 105–113.
Nagajyothi, P., Sreekanth, T., Lee, J. I., and Lee, K. D. (2014). Mycosynthesis: Antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. Journal of Photochemistry and Photobiology B, 130, 299–304.
Nikaido, H. (2009). Multidrug resistance in bacteria. Annual Review of Biochemistry, 78(1), 119–146.
Omdurman Islamic University et al. (2026). Macrolide resistance in Streptococcus pyogenes from tonsillitis patients: Phenotypic profiles, resistance genes, and clinical associations. Preprint. https://doi.org/10.21203/rs.3.rs-7929845/v1
Ong, W. T. J., and Nyam, K. L. (2022). Evaluation of silver nanoparticles in cosmeceutical and potential biosafety complications. Saudi Journal of Biological Sciences.
Pani, A., Lee, J. H., and Yun, S. I. (2016). Autoclave mediated one-pot-one-minute synthesis of AgNPs and Au–Ag nanocomposite from Melia azedarach bark extract with antimicrobial activity against food pathogens. Chemistry Central Journal, 10, 15.
Pereira, M. M. (2020). Metabolomic studies for the toxicity evaluation of nanoparticles used for biomedical purposes (Master's thesis, Universidade do Porto (Portugal)).
Pokhrel, L. R., Jacobs, Z. L., Dikin, D., and Akula, S. M. (2022). Five nanometer size highly positive silver nanoparticles are bactericidal targeting cell wall and adherent fimbriae expression. Scientific Reports, 12(1), 6729.
Salem, S. S., and Fouda, A. (2021). Green synthesis of metallic nanoparticles and their prospective biotechnological applications: An overview. Biological Trace Element Research, 199, 344–370.
Sanchooli, N., Sanchooli, E., and Khandan Barani, H. (2022). Investigating the effect of water filter made using polyurethane foam containing silver nanoparticles on controlling Yersinia ruckeri in Oncorhynchus. Iranian Journal of Fisheries Sciences, 21, 500–515.
Saravanan, A., Kumar, P. S., Karishma, S., Vo, D. V. N., Jeevanantham, S., Yaashikaa, P. R., et al. (2021). A review on biosynthesis of metal nanoparticles and its environmental applications. Chemosphere, 264, 128580.
Scimeca, M., Bischetti, S., Lamsira, H. K., Bonfiglio, R., and Bonanno, E. (2018). Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. European journal of histochemistry: EJH, 62(1), 2841.
Shafaghat, A. (2015). Synthesis and characterization of silver nanoparticles by phytosynthesis method and their biological activity. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 45(3), 381–387.
Souto, E. B., Silva, G. F., Dias-Ferreira, J., Zielinska, A., Ventura, F., Durazzo, A., (2020). Nanopharmaceutics: Part I—Clinical trials legislation and good manufacturing practices (GMP) of nanotherapeutics in the EU. Pharmaceutics, 12, 146.
Tolaymat, T. M., El Badawy, A. M., Genaidy, A., Scheckel, K. G., Luxton, T. P., and Suidan, M. (2010). An evidence-based environmental perspective of manufactured silver nanoparticle in syntheses and applications: A systematic review and critical appraisal. Science of the Total Environment, 408, 999–1006.
Vicidomini, C., Roviello, V., and Roviello, G. N. (2021). Molecular basis of the therapeutical potential of clove (Syzygium aromaticum L.) and clues to its anti-COVID-19 utility. Molecules, 26(7), 1880.
Vivsa, R., Barbosa, A. A. T., Dolabela, S. S., and Jain, S. (2019). Multidrug-resistant bacteria and alternative methods to control them: An overview. Microbial Drug Resistance, 25(6), 890–908.
Wangpraseurt, D., You, S., Sun, Y., and Chen, S. (2022). Biomimetic 3D living materials powered by microorganisms. Trends in Biotechnology.
Yeung, A. W. K., Souto, E. B., Durazzo, A., Lucarini, M., Novellino, E., Tewari, D., (2020). Big impact of nanoparticles: Analysis of the most cited nanopharmaceuticals and nanonutraceuticals research. Current Research in Biotechnology, 2, 53–63.
Zhang, J., Shi, W., Ma, Q., Cui, H., and Zhang, L. (2021). Application of nanotechnology in immunity against infection. Coatings, 11, 430.
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