EVALUATION OF GRA6 AS GENETIC MARKER FOR DETERMINING TOXOPLASMA GONDII GENOTYPE IN THE CEREBROSPINAL FLUID OF HIV/AIDS PATIENTS WITH TOXOPLASMIC ENCEPHALITIS

Authors

  • Nora HARMINARTI Universitas Andalas
  • IKA Puspa SARI Department of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • Wayan Tunas ARTAMA Departement of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Darma IMRAN Department of Neurology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • Agnes KURNIAWAN Department of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.21010/Ajid%20v19i1.4

Keywords:

GRA6, genetic marker, genotyping, cerebrospinal fluid, Toxoplasmosis encephalitis, PCR

Abstract

Background: Toxoplasmic encephalitis is a severe manifestation of Toxoplasma gondii infection, with potentially fatal outcomes, particularly among immunocompromised patients. Clinical manifestation of this infection is associated with a specific genotype of T. gondii, requiring the use of genetic marker for genotype determination.

Aims: This study critically evaluated the application of GRA6 gene as genetic marker for genotyping T. gondii in cerebrospinal fluid (CSF) samples from HIV/AIDS patients diagnosed with Toxoplasmic encephalitis.

Methods: The study analyzed 69 CSF samples from HIV/AIDS patients with Toxoplasmic encephalitis. These samples tested positive for Toxoplasma IgG serology and SAG2 PCR, while GRA6 genotyping was conducted using PCR-sequencing methods.

Results: The results showed that GRA6 had potential for genotyping in positive control settings from culture cells. However, there was limited effectiveness in CSF samples from Toxoplasmic encephalitis patients.

Conclusion: GRA6 had been proven effective as a genetic marker for the identification of T. gondii genotype among HIV/AIDS patients with Toxoplasmic encephalitis. However, the evaluation of GRA6 showed more effectiveness in cultured cells compared to direct clinical samples, such as cerebrospinal fluid obtained from HIV/AIDS patients with Toxoplasmic encephalitis.

 

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Published

2024-10-25

How to Cite

HARMINARTI, N., SARI , I. P., ARTAMA , W. T., IMRAN, D., & KURNIAWAN, A. (2024). EVALUATION OF GRA6 AS GENETIC MARKER FOR DETERMINING TOXOPLASMA GONDII GENOTYPE IN THE CEREBROSPINAL FLUID OF HIV/AIDS PATIENTS WITH TOXOPLASMIC ENCEPHALITIS . African Journal of Infectious Diseases (AJID), 19(1), 29–35. https://doi.org/10.21010/Ajid v19i1.4

Issue

Vol. 19 No. 1 (2025)

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Articles

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