Vol 16 No 1 (2022)


Katibi Oludolapo Sherifat
Dermatology Unit, Department of Paediatrics and Child health, University of Ilorin, Ilorin, Nigeria
Aboh Mercy Itohan
Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research & Development, Abuja, Nigeria
Salawu Oluwakayinsola Adeola
Department of Pharmacology & Toxicology, National Institute for Pharmaceutical Research & Development, Abuja, Nigeria.
Kola -Mustapha Adeola
Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical sciences, University of Ilorin, Ilorin, Nigeria
Olatunji Lawrence Aderemi
Department of Physiology, College of health Sciences, University of Ilorin, Ilorin, Nigeria.
Published December 21, 2021
  • Anti-fungal; Acalypha wilkesiana; skin diseases; ethnobotanical medicine
How to Cite
Sherifat, K., Itohan, A., Adeola, S., Adeola, K., & Aderemi, O. (2021). ANTI-FUNGAL ACTIVITY OF ACALYPHA WILKESIANA: A PRELIMINARY STUDY OF FUNGAL ISOLATES OF CLINICAL SIGNIFICANCE. African Journal of Infectious Diseases (AJID), 16(1), 21-30. https://doi.org/10.21010/Ajid.v16i1.4



Acalypha wilkesiana (AW), a popular medicinal plant has been used in traditional medicine to treat a variety of skin disorders including pityriasis versicolor and seborrheic dermatitis. As a prelude to clinical trials in humans, an experimental study was carried out to determine the spectrum of antifungal activity of 2 variants of the Acalypha wilkesiana plant.

Materials and Methods: The ethanol extract and herbal cream formulation of the dried leaves of 2 cultivars (Macrophylla & Hoffmani) of Acalypha wilkesiana were investigated for in-vitro antifungal activity by disc diffusion and micro-broth dilution techniques. Organisms tested were typed cultures of Malassezia furfur, Candida albicans and Trichophyton rubrum; and clinical strains of Microsporum canis and Epidermophyton floccosum.

Results: Both cultivars (Macrophylla and Hoffmanii) of the plant showed good activity against all the fungi tested except Microsporum canis (8.0±0.00; 7.00±0.00 mm). The greatest activity was observed against Trichophyton rubrum (22.0±0.00; 24.00±0.00 mm) while Candida albicans showed the least activity (15.0±0.00; 18.00±0.57 mm). The Minimum Inhibitory Concentration (MIC) of the crude extract ranged between 0.25 and 8 mg/ml for all organisms, while that of the herbal cream was 0.31-8mg/ml.  The lowest MIC was seen with Candida albicans for both varieties of the plant. The Acalypha wilkesiana Hoffmanii demonstrated a greater activity against Candida albicans and Malassezia furufur than the A. wilkesiana (Macrophylla).

Conclusion: This study reveals Acalypha wilkesiana leaf extract has potential for development as a cream that can be used to treat superficial fungal skin infections.