SIBLING PAIR ANALYSIS IN THE IDENTIFICATION PROCESS OF THE MADURESE POPULATION USING STR CODIS LOCI

Authors

  • Ahmad Yudianto 1Department of Forensic Medicine and Medicolegal-dr.Soetomo Hosptla, Faculty of Medicine
  • Arofi Kurniawan Department of Forensic Odontology, Faculty of Dental Medicine, Universitas Airlangga, Indonesia
  • Fery Setiawan Forensics Study Program, Magister Program of Postgraduate School, Universitas Airlangga, Indonesia
  • Egita Windrianatama Puspa Forensics Study Program, Magister Program of Postgraduate School, Universitas Airlangga, Indonesia
  • Racy Youngest Forensics Study Program, Magister Program of Postgraduate School, Universitas Airlangga, Indonesia

DOI:

https://doi.org/10.21010/Ajid%20v16i2.5

Keywords:

Identification, STR CODIS, Siblings, Madurese Population, Human & Mortality

Abstract

Background: The most common problem in forensic personal identification, particularly in paternity tests, is the availability of information originating from a mother or a father that can be used as a comparison in the forensic DNA examination process. The use of sibling analysis in paternity tests is still not widely known.

Materials and methods: The respondents of this study were 25 families of the Madurese population with 6, 9, and 10 sibling pairs categorized as female-female, male-male, and male-female siblings. The kinship relationship was analyzed by using 13 STR CODIS loci technique (TPOX, D3S1358, FGA, D5S818, CSFIPO, D7S820, D8S1179, THOI, vWA, D13S317, D16S539, D18S51, D21S11), and Amelogenin (Amel) x: 106bp,y: 112bp).

Results: The results of DNA contents and purity examination of the extracted DNA sample showed that the average value of DNA contents was 675±5.35 ng/μL with a purity range of 1.05-1.86. The findings proved that the male-male siblings of the Madurese population had the highest allele sharing percentage at the loci of D13S317, D16S539, and D21S11. The highest allele sharing percentage for female-female siblings was at the loci of TPOX and D21S11. Meanwhile, the male-female siblings had the highest allele sharing percentage at the loci of TPOX, D5S818, vWA, D7S820, THO1, vWA, and D13S317. 

Conclusion: The main STR loci recommended in the male-female siblings of Madurese population identification are TPOX, D13S317, and D21S11

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Published

2022-05-12

How to Cite

Yudianto, A., Kurniawan, A., Setiawan, F., Windrianatama Puspa, E., & Youngest, R. (2022). SIBLING PAIR ANALYSIS IN THE IDENTIFICATION PROCESS OF THE MADURESE POPULATION USING STR CODIS LOCI. African Journal of Infectious Diseases (AJID), 16(2), 42–54. https://doi.org/10.21010/Ajid v16i2.5

Issue

Vol. 16 No. 2 (2022)

Section

Articles

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