• Adetunji Joseph ALADESANMI Afe Babalola University, Ado Ekiti, Ekiti State Nigeria.
  • Ogu Emmanuel ODIBA Obafemi Awolowo University, Ile-Ife
  • Samuel Akintunde ODEDIRAN Obafemi Awolowo University, Ile-Ife
  • Ayodeji Oluwabunmi ORIOLA Obafemi Awolowo University



Background: The potential of Artocarpus altilis stem bark as a safe antimalarial agent, and the identification of its antimalarial constituents was explored.

 Materials and Methods:The air-dried stem bark was extracted with 70% ethanol, filtered and concentrated in vacuo to obtain the extract (EE). The extract was successively partitioned to give n-hexane (AAH),dichloromethane (AAD), ethyl acetate (AAE)n-butanol (AAB)and aqueous (AAQ)fractions respectively after determining the acute toxicity using Lorke’s method. These were each evaluated for chemosuppressive antimalarial activities (0-200mg/kg) against chloroquine-sensitive Plasmodium berghei-berghei-infected albino mice. Normal saline and chloroquine, 10 mg/kg were negative and positive control respectively.The survival times and percentage survivors of the mice in both experiments were determined after observation for twenty-eight days post-drug administration. The five (5) column chromatographic (CC) fractions, AAH1, AAH2, AAH3, AAH4andAAH5 obtained from the most active AAH, were also evaluated for antimalarial activities (0-50mg/kg). Further column purification and repeated PTLC of AAH5 yielded three bands, which were finally subjected to GC-MS analysis.

Results:EE gave ED50 and LD50 values of 227.17and >5000 mg/kg while its partitioned fractions gave ED50 values as follows: AAH, 79.14; AAD, 215.59;AAE, 160.46,AAB,81.42; and AAQ, 90.85 mg/kg respectively. The primary CC fractions also gave ED50 values as follows:AAH1 21.95;AAH2, 26.96;AAH3,21.30; AAH4, 20.92 andAAH5, 20.75 mg/kg respectively to identify AAH5 as the putative fraction. GC-MS analysis revealed eleven major compounds (1–11) in the three PTLC bands as the antiplasmodial constituents of the plant.

Conclusion:The stem bark of A. altilis is a potential agent in malaria control which is safe for oral use.

Author Biographies

Adetunji Joseph ALADESANMI, Afe Babalola University, Ado Ekiti, Ekiti State Nigeria.

Department of Pharmacognosy and Herbal Medicine, College of Pharmacy, Afe Babalola University, Ado Ekiti, Ekiti State Nigeria.


Ogu Emmanuel ODIBA, Obafemi Awolowo University, Ile-Ife

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo

University, Ile-Ife, Osun State, Nigeria

Research Student

Samuel Akintunde ODEDIRAN, Obafemi Awolowo University, Ile-Ife

Department of Pharmacognosy,

Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria

Senior Lecturer

Ayodeji Oluwabunmi ORIOLA, Obafemi Awolowo University

Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo

University, Ile-Ife, Osun State, Nigeria

Research Fellow


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How to Cite

ALADESANMI, A. J., Odiba, O. E., ODEDIRAN, S. A., & Oriola, A. O. (2022). ANTIPLASMODIAL ACTIVITIES OF THE STEM BARK EXTRACT OF ARTOCARPUS ALTILIS FORSBERG. African Journal of Infectious Diseases (AJID), 16(2), 33–45. v16i2S.5