Vol 13 No 2 (2019)
Articles

EXPRESSION OF IMUNOGLOBULIN, GRANZYME-B AND PERFORIN AGAINTS Ag85A AND Ag85B PROTEINS OF MYCOBACTERIUM TUBERCULOSIS IN BALB/C MICE

Fihiruddin Fihiruddin
Doctoral Program, Research Center of Biotechnology, Universitas Gadjah Mada, Yogyakarta. Indonesia
Wayan Tunas Artama
3Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta. Indonesia
Tri Wibawa
5Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta. Indonesia
Ni Made Mertaniasih
Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya. Indonesia
Published July 3, 2019
Keywords
  • Ag85A and Ag85B proteins, Imunoglobulin, granzym B and perforin, TB Vaccine
How to Cite
Fihiruddin, F., Artama, W., Wibawa, T., & Mertaniasih, N. (2019). EXPRESSION OF IMUNOGLOBULIN, GRANZYME-B AND PERFORIN AGAINTS Ag85A AND Ag85B PROTEINS OF MYCOBACTERIUM TUBERCULOSIS IN BALB/C MICE. African Journal of Infectious Diseases (AJID), 13(2), 13-20. https://doi.org/10.21010/Ajid.v13i2.2

Abstract

Background: Ag85 is a protein that may maintain survival of M.tuberculosis in intracellular parts of host cells and is considered as a virulence factor. The expression of Ag85 protein can stimulate proliferation and differentiation of B cells and T cells in patients with tuberculosis. This research aimed to determine the ability of Ag85A and Ag85B proteins in activating the response of antibodies, granzyme-B and perforin in Balb/c mice.Methods: 25 male Balb/c mice were assigned into five  groups. Group I was treated with adjuvant, group II with BCG vaccine (Biofarma), group III with a combination of BCG and Ag85A, group IV with a combination of BCG and Ag85B and group V with a combination of BCG, Ag85A and Ag85B. Concentrations of Immunoglobulin G, granzym-B and perforin were examined using ELISA and the number of CD8+ T-cells and NK T-cells were checked by flowcytometry.Results: The highest concentration of imunoglobulin G was found in group-V with 62.49±5.4327 ng/ml.  The highest mean number of CD8+ T-cells, NK T-cells, granzyme-B and perforin was found in group–IV with 4.32%, 1.03%, 35.11±1.7789 pg/ml and 6.19±0.2235 pg/ml respectively. The results of One-Way Anova test showed that there were significant differences in immunoglobulin responses, with p <0.05. The expressions of granzym-B and perforin were  higher in mice treated with combination of BCG and recombinant proteins.Conclusions: Ag85 protein can be combined with the BCG vaccine to improve protection against M. tuberculosis infection.