IN VITRO ANTIFUNGAL ACTIVITY AND CYTOTOXICITY SCREENING OF DRY CRUDE EXTRACTS FROM BRAZILIAN AMAZONIA PLANTS

Authors

  • Mônica Regina Pereira Senra Soares NUPITE, Faculdade de Odontologia, Universidade Federal de Juizde Fora; Rua José Lourenço, s/n, 36036-330 Juiz de Fora - Minas Gerais, Brazil;
  • César Augusto Caneschi
  • Maria das Graças Afonso Miranda Chaves
  • Marcela Mota
  • Pedro Henrique Fazza Stroppa
  • Wagner Barbosa
  • Nádia Rezende Barbosa Raposo NUPICS, Faculdade de Farmácia, Universidade Federal de Juiz de Fora; Rua José Lourenço, s/n, 36036-30 Juiz de Fora - Minas Gerais, Brazil,

DOI:

https://doi.org/10.21010/ajtcam.v15i4.2

Keywords:

Candidiasis, Natural products, Toxicity

Abstract

Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were evaluated. Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) 10231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy (SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts. Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis. Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis.

Author Biographies

Mônica Regina Pereira Senra Soares, NUPITE, Faculdade de Odontologia, Universidade Federal de Juizde Fora; Rua José Lourenço, s/n, 36036-330 Juiz de Fora - Minas Gerais, Brazil;

NUPITE, Faculdade de Odontologia, Universidade Federal de Juizde Fora; Rua José Lourenço, s/n, 36036-330 Juiz de Fora - Minas Gerais, Brazil;

Nádia Rezende Barbosa Raposo, NUPICS, Faculdade de Farmácia, Universidade Federal de Juiz de Fora; Rua José Lourenço, s/n, 36036-30 Juiz de Fora - Minas Gerais, Brazil,

NUPICS, Faculdade de Farmácia, Universidade Federal de Juiz de Fora; Rua José Lourenço, s/n, 36036-30 Juiz de Fora - Minas Gerais, Brazil

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Published

2018-10-16

How to Cite

Soares, M. R. P. S., Caneschi, C. A., Chaves, M. das G. A. M., Mota, M., Stroppa, P. H. F., Barbosa, W., & Raposo, N. R. B. (2018). IN VITRO ANTIFUNGAL ACTIVITY AND CYTOTOXICITY SCREENING OF DRY CRUDE EXTRACTS FROM BRAZILIAN AMAZONIA PLANTS. African Journal of Traditional, Complementary and Alternative Medicines, 15(4), 13–21. https://doi.org/10.21010/ajtcam.v15i4.2

Issue

Vol. 15 No. 4 (2018)

Section

Research Papers

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